Co-transformation to tobacco of Cre/lox site-specific recombination system and its precise recombination  

作　　者：Lei Li Songmei Liu Yuanlei Hu Zhongping Lin 

机构地区：[1]Peking Univ, Natl Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China

出　　处：《Chinese Science Bulletin》2000年第6期540-543,共4页

摘　　要：For the temporally and spatially regulated expression of the barnase gene in plant, two kinds of plasmids with ere gene and its directly repeat recognition sites lox from bacteriophage P1 were constructed and co-transformed into tobacco by agrobacterium mediated procedure. The transgenic plants were conformed by PCR analysis. The blocking fragment between the two lox directly repeat sites was excised by Cre protein in the transgenic plant genome. Cloning and seguencing the DNA fragment from the co-transformed plant DNA showed that the precise DNA excision occurred in transgenic tobacco genome directed by Cre//ox site-specif ic recombination .For the temporally and spatially regulated expression of the barnase gene in plant, two kinds of plasmids with cre gene and its directly repeat recognition sitesiox from bacteriophage P1 were constructed and co-transformed into tobacco by agrobacterium mediated procedure. The transgenic plants were conformed by PCR analysis. The blocking fragment between the twolox directly repeat sites was excised by Cre protein in the transgenic plant genome. Cloning and sequencing the DNA fragment from the co-transformed plant DNA showed that the precise DNA excision occurred in transgenic tobacco genome directed by Cre/lox site-specific recombination.

关 键 词：CRE/LOX SITE-SPECIFIC recombination system TOBACCO CO-TRANSFORMATION PRECISE recombination. 

分 类 号：S572[农业科学—烟草工业]