Neodymium cations Nd^(3+x) were transported to the interior of Euglena gracilis 277  

作　　者：KANG Lin, SHEN Zhiqiang & JIN Chengzhi Institute of Genetics, State key Laboratory of Genetic Engineering, Fudan University, Shanghai 200433, China Research Centre of Analysis and Measurement, Fudan University, Shanghai 200433, China Correspondence should be addressed to Jin Chengzhi 

出　　处：《Chinese Science Bulletin》2000年第7期585-592,共8页

摘　　要：Euglena gracilis 277, a unicellular green alga, demonstrated remarkable ability to transport Nd3+ to the cell compartments. For a given amount of Nd3+ and cells, the results of ICP-AES indicated that the cellular uptake of Nd3+ was independent of Nd3+ concentration in thebulk solution. The average uptake of Nd3+ per celi (mMd) is proportional to a parameter ξ-theratio of neodymium content to the celi counts of the system. A novel approach for probing cellular neodymium by tetraiodotetra chlorofluorescein (I4TCF) has been devised. Data derived from the cryosections of !4TCF-Nd3+ stained cells and EDAX of the fast freezing ultrathin cryosections indicate that Nd3+ is distributed over the celi compartments. Chloroplasts are the major compartments as the residence of Nd3+ in the alga. The transport should be against a concentration gradient of Nd3+ on the order of five, even higher. it is proposed that a calcium ion channel would play an important role in the Nd3+ transportation.Euglena gracilis 277, a unicellular green alga, demonstrated remarkable ability to transport Nd3+ to the cell compartments. For a given amount of Nd3+ and cells, the results of ICP-AES indicated that the cellular uptake of Nd3+ was independent of Nd3+ concentration in thebulk solution. The average uptake of Nd3+ per celi (mMd) is proportional to a parameter ξ-theratio of neodymium content to the celi counts of the system. A novel approach for probing cellular neodymium by tetraiodotetra chlorofluorescein (I4TCF) has been devised. Data derived from the cryosections of !4TCF-Nd3+ stained cells and EDAX of the fast freezing ultrathin cryosections indicate that Nd3+ is distributed over the celi compartments. Chloroplasts are the major compartments as the residence of Nd3+ in the alga. The transport should be against a concentration gradient of Nd3+ on the order of five, even higher. it is proposed that a calcium ion channel would play an important role in the Nd3+ transportation.

关 键 词：NEODYMIUM CATIONS (Nd³⁺) EUGLENA GRACILIS EDAX I4TCF. 

分 类 号：Q44[生物学—生理学]