Coat protein promoter from cotton leaf curl virus is not a tissue-specifically expressed promoter  

作　　者：XIE Yingqiu ZHU Zhen LIU Yule WU Qian XU Honglin 

机构地区：[1]Institute of Genetics,Chinese Academy of Sciences,Beijing 100101,China [2]Institute of Microbiology,Chinese Academy of Sciences,Beijing 100080,China

出　　处：《Chinese Science Bulletin》2000年第20期1869-1874,1921-1922,共8页

摘　　要：Geminivirus is a kind of single-stranded DNA virus. Experimental results from tomato golden mosaic virus (TGMV) showed that expression pattern of coat protein gene (cp) promoter was phloem specifically expressed. In this note, the studies on cp promoter of cotton leaf curl virus (CLCuV) which is found and identified recently suggest that the promoter is not phloem specifically expressed. The expressing activity of gus gene driven by the promoter exists not only in phloem but also in mesophyll tissues and root tip meristem. Transient expression suggests that cp promoter transactivated by AC2 shows expressing activity in mesophyll and vascular tissue of leaf vein.Geminivirus is a kind of single-stranded DNA virus. Experimental results from tomato golden mosaic virus (TGMV) showed that expression pattern of coat protein gene (cp) promoter was phloem specifically expressed. In this note, the studies oncp promoter of cotton leaf curl virus (CLCuV) which is found and identified recently suggest that the promoter is not phloem specifically expressed. The expressing activity ofgus gene driven by the promoter exists not only in phloem but also in mesophyll tissues and root tip meristem. Transient expression suggests thatcp promoter transactivated by AC2 shows expressing activity in mesophyll and vascular tissue of leaf vein.

关 键 词：CLCuV PROMOTER GUS TOBACCO cotton. 

分 类 号：S435.62[农业科学—农业昆虫与害虫防治]