Binding equilibrium of I^- to serum albumin with resonance Rayleigh scattering  

作　　者：梁宏 沈星灿 蒋治良 何锡文 申泮文 

机构地区：[1]Institute of Bioinorganic Chemistry, Chemistry and Life Science College, Guangxi Normal University, Guilin 541004, China [2]Department of Chemistry, Nankai University, Tianjin 300071, China

出　　处：《Science China Chemistry》2000年第6期600-608,共9页中国科学（化学英文版）

摘　　要：The binding equilibrium between l? and human serum albumin (HSA) or bovine serum albumin (BSA) has been studied by means of the resonance Rayleigh scattering (RRS) and equilibrium dialysis. It has been found for the first time that RRS and multiple frequency scattering (MFS) are enhanced as the l? binding to the HSA and BSA, but fluorescence quenches. The equilibrium dialysis results suggest that the binding of l? to HSA and BSA fits a phase-distribution model other than Scatchard model, and that the order of magnitude of its phase-distribution constant was found to be 104. It is most probable that Cl? or other anion ions influence the binding of l? by changing the ionic strength in the solution. The dialysis at different pH indicates that the binding mechanism is due to the electrostatic forces between the l? and protonated basic amino-acid residues.The binding equilibrium between l- and human serum albumin (HSA) or bovine serum albumin (BSA) has been studied by means of the resonance Rayleigh scattering (RRS) and equilibrium dialysis. It has been found for the first time that RRS and multiple frequency scattering (MFS) are enhanced as the l- binding to the HSA and BSA, but fluorescence quenches. The equilibrium dialysis results suggest that the binding of l- to HSA and BSA fits a phase-distribution model other than Scsitchard model, and that the order of magnitude of its phase-distribution constant was found to be 104. It is most probable that Cl~ or other anion ions influence the binding of P by changing the ionic strength in the solution. The dialysis at different pH indicates that the binding mechanism is due to the electrostatic forces between the T-and protonated basic amino-acid residues.

关 键 词：serum ALBUMIN IODINE ion resonance RAYLEIGH scattering EQUILIBRIUM DIALYSIS phase distribution. 

分 类 号：O657.3[理学—分析化学]