Introduction of a rice blight resistance gene, Xa21, into five Chinese rice varieties through an Agrobacterium -mediated system  

作　　者：翟文学 李晓兵 田文忠 周永力 潘学彪 曹守云 赵显峰 赵彬 章琦 朱立煌 

机构地区：[1]Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China [2]Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, Beijing 100080, China [3]Agricultural College, Yangzhou University, Yangzhou 225009, China

出　　处：《Science China(Life Sciences)》2000年第4期361-368,共8页中国科学（生命科学英文版）

摘　　要：A cloned gene, Xa21 was transferred into five widely-used Chinese rice varieties through an Agrobacterium-mediated system, and over 110 independent transgenic lines were obtained. PCR and Southern analysis of transgenic plants revealed the integration of the whole Xa21 gene into the host genomes. The integrated Xa21 gene was stably inherited, and segregated in a 3 : 1 ratio in the selfed T1 generation when one copy of the gene was integrated in the transfor-mants. Inoculation tests displayed that transgenic T0 plants and Xa21 PCR-positive T1 plants were highly resistant to bacterial blight disease. The selected Xa21 homozygous resistant transgenic lines with desirable qualities may be propagated as new varieties or utilized in hybrid rice breeding.A cloned gene, Xa21 was transferred into five widely-used Chinese rice varieties through an Agrobacterium-mediated system, and over 110 independent transgenic lines were obtained. PCR and Southern analysis of transgenic plants revealed the integration of the whole Xa21 gene into the host genomes. The integrated Xa21 gene was stably inherited, and segregated in a 3 : 1 ratio in the selfed T1 generation when one copy of the gene was integrated in the transfor-mants. Inoculation tests displayed that transgenic T0 plants and Xa21 PCR-positive T1 plants were highly resistant to bacterial blight disease. The selected Xa21 homozygous resistant transgenic lines with desirable qualities may be propagated as new varieties or utilized in hybrid rice breeding.

关 键 词：transformation XA21 RICE AGROBACTERIUM blight disease resistance. 

分 类 号：S435.121[农业科学—农业昆虫与害虫防治]