三七皂苷对缺血大鼠脑组织神经元型一氧化合酶的调节作用  

Effect of notoginseneside on the expression abundance of neuronal nitric oxide synthase in cerebral cortex, hippocampus, and caudate putamen nuclei during incomplete cerebral ischemia in old rats

作　　者：邹玉安[1] 王家鑫[2] 魏阿平[1] 王增义 龙卿[1] 张九鸿[1] 

机构地区：[1]张家口医学院第一附属医院神经内科,075000 [2]张家口农业高等专科学校组织学与胚胎学教研室,075131 [3]宣化钢铁公司职工医院,075100

出　　处：《神经药理学报》2000年第2期6-9,共4页Acta Neuropharmacologica

基　　金：河北省科学技术研究与发展计划基金

摘　　要：目的:研究三七皂苷保护缺血脑组织中神经元的机制。方法:应用免疫细胞化学SP法和计算机图象分析系统观察三七皂苷(7mg.kg-1,i.m) 对不同缺血时间(4,12和24h)老龄大鼠大脑皮质、海马与尾壳核内神经元型一氧化氮合酶(nNOS)表达丰度和病理组织学变化的影响。结果:单侧结扎颈总动脉可使大脑皮层、海马与尾壳核内nNOS的表达丰度显著下降,部分神经元出现空泡样变性。预先注射三七皂苷可有效阻止大脑皮质、海马与尾壳核内nNOS表达丰度的下降,并减少这些区域的病理学改变。结论:三七皂苷可促进缺血早期脑组织表达nNOS,并由此对神经元发挥保护作用。OBJECTIVE:To investigate and consider the protective mechanism of notoginsenoside on neurons in ischemic brains. METHODS: Immnocytochemistry and computer image analysis system were employed to detect the expression abundance of neuronal nitric oxide synthase(nNOS) in cerebral cortex, hippocampus, and caudate puta-men nuclei from a Rat model of incomplete cerebral ischemia at different timepoints of unilateral common carotid artery occlusion(4, 12, and 24hJ with i.m. injection of notoginsenoside 4h prior to ischemic model replication. At the same time, histopathological changes of neuronal damage were evaluated by utilizing the H & E staining procedure. RESULTS:The expression abundance of nNOS in cerebral cortex, hippocampus, and caudate putamen nuclei from the rats with unilateral common carotid artery being ligated decreased. Some neurons on the slices prepared from isdemic cerebral cortex, hippocampus, and caudate putamen nuclei appeared to be vacuolar degeneration. Pretreatment decreases of nNOS and reduce the extent of neuronal damage induced by ischemia. CONCLUSION: Notoginsenoside may play a newroprotective role in incomplete cerebral ischemia through upregulating the expression of nNOS in cerebral cortex. hippocampus, and caudate putamen nuclei.

关 键 词：三七皂苷 临缺血 神经元型一氧化氮合酶 大鼠 

分 类 号：R285[医药卫生—中药学]