补肾中药复方对小鼠精子受精能力影响的研究  

Influence of Compound Chinese Medicine for Reinforcing Kidney on Mouse Sperm Fertilizing Ability

作　　者：蒋小辉[1] 张伦[1] 梁鑫[1] 胡翔[2] 陆华[1] 

机构地区：[1]成都中医药大学第二临床医学院生殖医学实验室,成都610041 [2]成都中医药大学,成都610075

出　　处：《四川大学学报（医学版）》2011年第6期789-791,796,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家重点基础研究发展计划(编号2010CB530403);成都中医药大学校基金(编号ZRYB200951;ZRYY201027)资助

摘　　要：目的观察补肾中药复方对小鼠精子受精能力的影响,并探讨其作用机制。方法正常雄性小鼠随机分为服用补肾中药复方的实验组和服用生理盐水的对照组,实验组灌服补肾中药复方溶液,1次/d,每次1mL,共14d,对照组同时间灌服等量的生理盐水。于末次灌胃后第7d与超排的雌鼠合笼,合笼成功后处死雌鼠取出受精卵(1cell胚),体内实验观察卵裂率和囊胚生成百分率;体外实验观察体外受精百分率(IVF%)和囊胚率,并观察与精子受精能力相关的完整顶体百分率(AIS%)和顶体酶活性。结果体内实验发现实验组卵裂率(88.3%±1.29%)高于对照组(62.1%±3.48%),P=0.005;体外实验发现实验组IVF%为82.4%±2.89%,高于对照组(62.5%±3.58%),P=0.015;而体内、外实验均发现两组的囊胚生成百分率差异无统计学意义。实验组顶体酶活性为(44.29±5.49)IU,与对照组〔(27.12±4.87)IU〕的差异有统计学意义(P=0.048),两组AIS%差异无统计学意义。结论补肾中药复方能提高小鼠精子的受精能力,其作用机制有可能通过调节顶体酶活性来影响小鼠精子的受精能力。Objective To study the influence of Compound Chinese medicine for reinforcing kidney on sperm fertilizing ability of mice.Methods Twenty male mice were randomly divided into two groups.The mice in treatment groups were given Compound Chinese medicine for reinforcing kidney every day for 14 d,and the mice in control group were given saline.Seven days after the treatments,the male mice mated with superovulated female mice.After the successful mating,the female mice were executed,then 1 cell embryos and fertilized eggs were harvested from oviduct.The embryos cleavage rate and the percentage rate of blastocysts were observed in vivo.In vitro fertilization（IVF%）,the percentage of blastocysts,the percentage of acrosome-intact spermatozoa（AIS%） and the acrosin activity were studied in vitro.Results Compared with the controls,the mice receiving Compound Chinese medicine had higher embryos cleavage rate and IVF %.The percentage of blastocysts between the two groups was no difference.The acrosin activity of treated mice was significant higher than that of control mcie,and AIS% between the two groups was no significant different.Conclusion Compound Chinese medicine for reinforcing kidney can improve the mouse sperm fertilizing ability,which may be relevant to acrosin activity.

关 键 词：补肾中药复方 精子受精能力 顶体酶活性 

分 类 号：R285.5[医药卫生—中药学]