两株野生鹅膏菌水相多糖的体外抗肿瘤细胞活性研究  

Antitumor Activity of Polysaccharides Extracted from Two Wild Amanitas

作　　者：唐瑗璘[1] 罗强[1] 丁文[1] 丁祥[1] 杨志荣[1] 

机构地区：[1]四川大学生命科学学院资源微生物与生物技术四川省重点实验室,成都610065

出　　处：《四川大学学报（医学版）》2011年第6期792-796,共5页Journal of Sichuan University(Medical Sciences)

摘　　要：目的确定两株野生鹅膏菌体外抗肿瘤活性的有效成分,并测定有效成分对肿瘤细胞的体外抑制率。方法从四川地区采集的两株野生鹅膏菌子实体,结合形态学和分子生物学对两株菌进行分类鉴定;应用石油醚、氯仿、甲醇和水对鹅膏菌子实体进行萃取,用MTT方法测定所得组分对人胃癌细胞(MGC)和人结肠癌细胞(Lovo)的体外抑制效果;分析水相提取物主要成分。水提法制备多糖,测定所得多糖体外对肿瘤细胞的抑制率。结果经鉴定两株野生鹅膏菌分别为黄盖鹅膏(Amanita subjunquillea)和豹斑鹅膏(Amanita pantherina)。两株野生鹅膏菌子实体水相提取物对肿瘤细胞抑制具有较好的效果,水相提取物主要成分为多糖。黄盖鹅膏菌的多糖对MGC细胞IC50值为3.790mg/mL。结论两种鹅膏菌提取多糖对MGC胃癌细胞有较好的生长抑制效果,具有成为抗肿瘤药物的潜力。Objective To investigate the anti-tumor activity of two wild Amanita strains,and to determine the inhibition ratio of their effective chemical components on tumor cells in vitro.Methods Two wild Amanita strains collected in Sichuan province were identified through morphology and molecular methods.The chemical components from the fruit body of the two strains were extracted with petroleum ether,chloroform,methanol and water.The anti-tumor activity of the extracts were tested on the cancer cells of MGC and Lovo strains.The polysaccharides were isolated with hot water and tested for its anti-tumor activity in vitro.Results The two wild Amanitas were identified as Amanita subjunquillea and Amanita pantherina.The water-soluble extracts from the Amanitas had anti-tumor effect,with polysaccharide as one of the main effective components.The IC50 value of the polysaccharide isolated from M1 was 3.790 mg/mL for the MGC cells.Conclusion Polysaccharides isolated from the two wild Amanitas have considerable anti-tumor activity against MGC cells in vitro.

关 键 词：鹅膏菌 多糖 抗肿瘤 

分 类 号：R285.5[医药卫生—中药学]