Clearance of increased serum advanced glycosylation end products in patients with end stage renal disease by hemodialysis  

血液透析清除终末期肾病患者增高的血清高级糖基化终产物(英文)

作　　者：刘乃丰[1] 孙子林[1] 刘必成[1] 

机构地区：[1]南京铁道医学院第一附属医院心内科

出　　处：《Acta Pharmacologica Sinica》2000年第3期86-90,共5页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China, № 39470314.

摘　　要：AIM: To study the effect of removal of hemodialysis using acetate membrane on serum advanced glycosylation end products (AGEP) in 36 patients (59.1±1.6) with end stage renal disease (ESRD). METHODS: Serum AGEP levels were determined with quantitative fluorescence spectrometry, flow injection analysis (FIA), and competitive enzyme-linked imrnunosorbent assay (ELISA) using a polyclonal antibody directed against AGEP. RESULTS: The serum AGEP levels in patients with ESRD quantified by fluorescence spectrometry, FIA, and ELISA were higher than those in controls [(25±5) vs (7.5±1.5), (6.1±1.8) vs (1.4±0.5), and (37±20) vs (9±10) kU/L, respectively] and markedly reduced to [(22±6), (4.2±1.4), and (19±14) kU/L, respectively] after hemodialysis. CONCLUSION : Increased serum AGEP levels in the circulation of patients with ESRD were reduced effectively by hemodialysis. advanced glycosylation end-products; kidney diseases; flow injection analysis; enzyme-linked immunosorberit assay; fluorescence spectrometry; hemodialysis目的:研究血液透析对36例终末期肾病患者血中高级糖基化终产物的清除效果。方法:分别用荧光分光光谱、流动注射分析法和酶联免疫吸附法测定终末期肾病患者血中高级糖基化终产物的水平及比较血液透析前后的变化。结果:荧光分光光谱、流动注射法和酶联免疫吸附法测定的终末期肾病患者血中高级糖基化终产物的水平显著高于对照组,分别为(25±5)vs(7.5±1.5),(6.1±1.8)vs(1.4±0.5)和(37±20)vs(9±10)kU/L,透析后上述水平分别降到(22±6),(4.2±1.4)和(19±14)kU/L。结论:血液透析能有效地清除终末期肾病患者血中增加的高级糖基化终产物。

关 键 词：advanced glycosylation end-products kidney diseases flow injection analysis enzyme-linked immunosorbent assay fluorescence spectrometry HEMODIALYSIS 

分 类 号：R692.5[医药卫生—泌尿科学]