Free radical scavenging and inhibition of lipid peroxidation by magnesium lithospermate B  

丹酚酸B镁盐对自由基的清除作用和对脂质过氧化的抑制作用(英文)

作　　者：吴兴军[1] 王逸平[1] 王唯 孙伟康 徐亚明 宣利江 

机构地区：[1]中国科学院上海药物研究所

出　　处：《Acta Pharmacologica Sinica》2000年第9期89-92,共4页中国药理学报（英文版）

基　　金：Project supported by the special subject of Bioscience & Biotechnology Research, Chinese Academy of Sciences, No STZ-1-09.

摘　　要：AIM: To study the effect of magnesium lithospermate B (MLB) on the lipid peroxidation and on its free radical scavenging activity. METHODS; MLB was incubated in rat tissue homogenate or in a free radical generating system. MLB induced inhibition of lipid peroxidation and its scavenging activity on superoxide anions and hy-droxyl radicals was studied using colorimetric estimation. RESULTS: MLB inhibited the lipid peroxidation induced by either an auto-oxidant or Fe2+/VitC in vitro, in the liver homogenate, the inhibitory rate of MLB (10 mg/L) being 69.2 % and 57.7%, respectively. MLB (25 and 50 mg/kg ) decreased the amount of thiobarbituric acid reactive substances (TBARS) in rat serum, liver, kidney, and heart. However, it did not inhibit the lipid peroxidation of brain homogenate ex vivo. MLB scav-enged superoxide anions generated from xanthine/xan-thine oxidase system and iron-dependent hydroxyl radi-cals. CONCLUSION: MLB is an inhibitor of lipid peroxidation and scavenge superoxide anions and hydroxyl radicals both in vitro and ex vivo.目的:研究丹酚酸B镁盐对过氧化脂质生成的影响和对自由基的清除作用.方法:不同浓度的丹酚酸B镁盐与大鼠组织匀浆或自由基发生系统共浴后,比色法观察它对过氧化脂质生成的抑制作用和对自由基的清除作用.结果:丹酚酸B镁盐抑制组织匀浆的脂质自氧化或硫酸亚铁和维生素C激发的脂质过氧化.在肝组织匀浆,丹酚酸B镁盐10 mg/L对两者的抑制率分别是69.2％,57.7％.丹酚酸B镁盐可清除黄嘌呤/黄嘌呤氧化酶系统产生的氧阴离子和Fe^(2+)-H_2O_2系统产生的羟自由基.结论:丹酚酸B镁盐能抑制脂质过氧化并对自由基有一定的清除作用.

关 键 词：Salvia miltiorrhiza magnesium lithos-permate B hydroxyl radical SUPEROXIDES lipid peroxi-dation 

分 类 号：R96[医药卫生—药理学]