Long-term outcome of acute renal injury induced by Aristolochia manshuriensis Kom in rats  

木通所致大鼠急性肾损伤的远期效应(英文)

作　　者：裘奇[1] 刘志红[1] 陈惠萍 印洪林 黎磊石 

机构地区：[1]南京大学医学院临床学院

出　　处：《Acta Pharmacologica Sinica》2000年第12期74-80,共7页中国药理学报（英文版）

摘　　要：AIM: To investigate the long-term functional and morphological changes of the kidney induced by acute intoxication of Aristolochia manshuriensis Kom in rats. METHODS: Experimental model of acute renal injury was established in the Sprague-Dawley rats with oral administration of decoctions of Chinese herb, Aristolochia manshuriensis Kom, at dosages of 50 g·kg-1·d-1 and 30 g·kg-1·d-1 for 7 consecutive days, and 20 g·kg-1·d-1 for 15 consecutive days. Renal function was assayed at months 0 (right after treatment) ,1,3, and 6 of the experiment. Renal histological examination was also performed. RESULTS: 1) At month 0, the renal functional changes of acute renal injury included azotemia, low molecular weight proteinuria, glycosuria, hypoosmotic urine, and NAG enzymuria. Histopatho-logical changes showed acute tubular necrosis, predominantly at the corticomedullary junction. 2) At months 1 and 3, the renal function of rats of the experiment was gradually restored and histopathologic examination suggested that the tubular lesions gradually recovered. In HE sections, basophilic deposits were observed in the tubular cytoplasm. And interstitial infiltration of inflammatory cells was not prominent. 3) At months 6, renal preneoplastic lesions, renal tumors, and extrarenal tumors occurred in rats. The occurrence of renal preneoplastic lesions at dosages of 50 g·kg-1·d-1, 30 g·kg-1·d-1,and 20 g·kg-1·d-1 were 100.0 % at all three doses, renal tumors 42.8 %, 25.0 %, and 0 % , respectively, extrarenal tumors 14.4 %, 12.5 %, and 12.5 %, respectively, and systemic tumors 57.2 %, 37.5 %, and 12.5 % , respectively, The occurrence of basophilic deposits, renal preneoplastic lesions, renal tumors, and extrarenal tumors in normal control group was nil. CONCLUSIONS:1) Administration of large dosage of Aristolochia manshuriensis Kom induces acute renal failure in rats. 2) The long-term renal function and histopathologic changes of acute renal injury induced by Aristolochia manshuriensis Komrecover spontaneously. 3) Aristolochia目的:观察木通所致大鼠急性肾损伤的远期效应(功能及组织形态学改变特点).方法:用不同剂量木通水煎剂(50 g·kg^(-1)·d^(-1)×7 d、30 g·kg^(-1)·d^(-1)×7d、20 g·kg^(-1)·d^(-1)×15 d)给大鼠灌胃,建立大鼠急性肾损伤动物模型后,检测实验开始(给药结束之日)、1月、3月、6月时各种肾功能指标,及肾组织形态学的变化.结果:1)木通所致大鼠急性肾损伤的近期功能改变为,氮质血症、低渗尿、低分子蛋白尿、糖尿、尿NAG酶升高.组织形态学改变主要为急性肾小管坏死,以皮髓交界区小管损伤为主.2)实验1月、3月木通组大鼠的肾功能逐渐恢复,皮髓交界区小管病变逐渐恢复,小管上皮细胞胞浆内可见嗜碱性物质沉积,间质浸润细胞较少,未见间质慢性炎症和纤维化等慢性化病变.3)实验6月大鼠发生肾脏间质肿瘤样增生及全身性肿瘤.结论:1)大剂量木通可导致大鼠急性肾功能衰竭.2)木通所致大鼠急性肾损伤远期其肾功能及小管损伤可逐渐恢复,未见明显小管间质慢性化病变.3)木通具有致肿瘤作用.

关 键 词：Aristolochia manshuriensis Kom NEPHROTOXICITY acute renal failure interstitial nephritis kidney diseases NEOPLASMS 

分 类 号：R285[医药卫生—中药学]