Effect of batroxobin against dog heart ischemia/reperfusion injury  

巴曲酶对抗狗心脏缺血/再灌损伤(英文)

作　　者：姜志胜[1] 夏春芳[1] 田青萍 符民桂[1] 王晓红[1] 庞永政[1] 唐朝枢[1] 刘乃奎[1] 

机构地区：[1]北京医科大学心血管基础所

出　　处：《Acta Pharmacologica Sinica》2000年第1期72-76,共5页中国药理学报（英文版）

摘　　要：AIM: To study the effect of batroxobin(Bat) on dog heart ischemia/reperfusion (I/R) injury. METHODS: Dog heart I/R injury was induced by occluding the left anterior descending coronary artery for 30 min and restoring blood perfusion for 90 min. Bat was intravenously injected before heart ischemia and 15 min before reperfusion. Plasma creatine kinase (CK), lac-tate dehydrogenase (LDH), and myocardial malondi-adehyde (MDA) concentrations were measured. The pathologic changes of I/R myocardium were observed. RESULTS: Bat reduced the mortality rate of I/R dog (I/R group 65.0 % vs Bat-Ⅰ group 30.0 % and Bat-Ⅱ group 28.6%, P < 0.05). Myocytes of I/R heart showed intracellular edema, damaged mitochondria, and concentrated nucleus. Bat decreased these changes. In Bat-Ⅰ and Bat- Ⅱ group, plasma CK and LDH level were reduced, the + dp/dtmax and - dp/ dtmax at 30 min after ischemia and 90 min after reperfusion were elevated , and left ventricular end dilation pressure ( LVEDP) was lowered. The myocardial MDA contents were decreased by 42.3 % and 38.1 % (P<0.01) in Bat-Ⅰ and Bat-Ⅱ group, respectively. CONCLUSION: Bat may exert an apparent role against dog heart ischemia/reperfusion injury and improve myocardial function.目的:研究巴曲酶(Bat)对狗心脏缺血/再灌损伤的影响。方法:狗冠脉左前降枝结扎30 min后恢复血液灌注,于缺血前(Bat-Ⅰ组)或缺血后再灌前15min(Bat-Ⅱ组)静脉注射Bat(1 Bu·kg^(-1))。测定dp/dt_(max)和LVEDP及血浆CK和LDH及心肌MDA含量,观察心肌病理形态学改变。结果:I/R组动物缺血或再灌后死亡率高达65.0％,心肌损伤明显。Bat-Ⅰ和Bat-Ⅱ组动物的死亡率分别为30.0％和28.6％,P<0.05,心肌损伤减轻;血浆CK、LDH含量,LVEDP及心肌MDA含量降低;+dp/dt_(max)和-dp/dt_(max)增加。结论:Bat可明显拮抗狗心脏缺血/再灌注损伤,改善心功能。

关 键 词：REPTILASE myocardial reperfusion injury heart function tests lactate dehydrogenase crea-tine kinase MALONDIALDEHYDE electron microscopy 

分 类 号：R96[医药卫生—药理学]