Influence of batroxobin on cerebral ischemia-reperfusion injury in gerbils  

巴曲酶对沙土鼠脑缺血再灌注损伤的影响(英文)

作　　者：陈群[1] 曾因明[1] 许鹏程[2] 范建伟[2] 

机构地区：[1]徐州医学院附属医院麻醉科 [2]徐州医学院江苏省麻醉学重点实验室

出　　处：《Acta Pharmacologica Sinica》2000年第2期67-70,共4页中国药理学报（英文版）

基　　金：Project supported by the grant of Jiangsu Provincial Health Bureau, No Z9717.

摘　　要：AIM: To study the effects of batroxobin (Bat) on neurons survival, neurobehavioral test, ATP levels and hy-droxyl radical outputs in hippocampus during forebrain is-chemia-reperfusion in gerbils. METHODS: The fore-brain ischemia was induced by occluding the bilateral common carotid arteries for 10 min in gerbils, and ATP levels and 2,3-dihydroxybenzoic acid (DHBA) outputs were assayed by HPLC. The neurons survival were assessed by histology, and behavioral tests of gerbils were assessed by open field test. RESULTS: The number of neurons survival in Ir at d 7 postischemic insult were (7±4) % of sham-operated gerbils, much less than that in Bat (45±16) % . The levels of explore activities of is-chemic gerbils was 175 % and 159 % of sham-operated gerbils at d 3 and d 6 postischemic insult, much more than that in Bat (120 % d 3 and 140 % d 6). Hip-pocampal ATP levels in Ir were 64 % of sham-operated gerbils at reperfusion 60 min, much less than that in BatⅠ and Ⅱ (82 % and 89 % respectively). The hip-pocampal 2,3-DHBA outputs in Ir increased by 4.5 folds of sham-operated gerbils at reperfusion 60 min, but the 2,3-DHBA outputs in Bat Ⅰ and Bat Ⅱ were only 2.6 and 2.4 folds respectively. CONCLUSION: Bat possesses the inhibitory effects on DND and OH production following cerebral ischemia-reperfusion in gerbils.目的:研究巴曲酶对沙土鼠脑缺血再灌注(Ir)期间海马存活锥体细胞数目,神经行为学,海马ATP和羟自由基含量的影响。方法:阻断沙土鼠双侧颈总动脉造成前脑缺血10min。高效液相测定海马ATP和2,3-DHBA的含量。结果:Ir组沙土鼠脑缺血后d7存活锥体细胞数(7±4)％少于Bat组的(45±16)％。在脑缺血后d3和d6时,Ir组沙土鼠的探究活动次数多于Bat组的120％和140％。在再灌注60min时,Ir组沙土鼠ATP水平低于Bat Ⅰ和Ⅱ组(82％和89％),但2,3-DHBA的含量高于Bat Ⅰ和Ⅱ组(2.6和2.4倍)。结论:巴曲酶具有减少脑缺血再灌注后延迟性神经元死亡和羟自由基产生的作用。

关 键 词：cerebral ischemia BRAIN adenosine triphosphate free radicals REPTILASE maze learning GERBILLINAE 

分 类 号：R96[医药卫生—药理学]