Anti-HIV-1 activity of trichobitacin, a novel ribosome-inactivating protein  

具有抗HIV-1活性的新核糖体失活蛋白-栝楼蛋白(英文)

作　　者：郑永唐[1] 贲昆龙[1] 金善炜[2] 

机构地区：[1]中国科学院昆明动物研究所 [2]中国科学院上海有机化学研究所

出　　处：《Acta Pharmacologica Sinica》2000年第2期85-88,共4页中国药理学报（英文版）

基　　金：Project supported by Chinese Academy of Sciences, Applied and Basic Research Foundation of Yunnan No 96CO95M

摘　　要：AIM: To determine whether trichobitacin, a novel ribo-some-inactivating protein purified from the root tubers of Trichosanthes kirilowii, possesses the anti-HIV activity. METHODS: The inhibition of syncytial cell formation induced by human immunodeficiency virus type Ⅰ (HIV-Ⅰ) was determined under microscope, reduction of HIV-Ⅰ p24 antigen expression level was measured by ELISA, and decrease in numbers of HIV-Ⅰ antigen positive cells in acutely and chronically infected cultures were detected by indirect immunofluorescence assay. RESULTS: Trichobitacin was found to greatly suppress syncytial cell formation induced by HIV-Ⅰ and to markedly reduce both expression of HIV-Ⅰ p24 antigen and the number of HIV antigen positive cells in acutely but not chronically HIV-Ⅰ infected culture. The median inhibitory concentration (IC50) in inhibition of syncytial cell formation and HIV antigen positive cells were 5 μg·L-1 (95 % confidence limits: 1.3-20μg·L-1) and 0.09 mg·L-1(95 % confidence limits: 0.011 - 0.755 mg·L-1), respectively. CONCLUSION: Trichobitacin is a novel ribosome-inactivating protein with anti-HIV-Ⅰactivity.目的:研究新核糖体失活蛋白-栝楼蛋白是否具有抗HIV-1活性。方法:合胞体抑制实验以光镜检查,以ELISA测定HIV-1 p24抗原表达水平,用间接免疫荧光技术测定HIV抗原阳性细胞的百分率。结果:栝楼蛋白对HIV-1诱导C8166细胞形成合胞体有显著的抑制作用。栝楼蛋白显著地抑制了HIV-1急性感染T细胞中p24核心抗原的表达水平和减少了HIV抗原阳性细胞的百分率。栝楼蛋白抑制合胞体形成和HIV抗原阳性细胞的IC_(50)分别是5μg·L^(-1)和0.09mg·L^(-1)。栝楼蛋白不影响慢性感染T细胞中HIV-1 p24抗原表达水平。结论:栝楼蛋白是具有抗HIV-1活性的新的核糖体失活蛋白。

关 键 词：trichobitacin protein synthesis inhibitors HW-1 anti-HIV agents plant proteins giant cells HIV antigens 

分 类 号：R96[医药卫生—药理学]