A Surface Plasmon Resonance-based Immunosensors for Sensitive Detection of Heroin  

作　　者：吴仲城 陈文革 王连超 戈瑜 虞承端 方廷健 

出　　处：《Plasma Science and Technology》2000年第6期581-587,共7页等离子体科学和技术（英文版）

摘　　要：A simple technique for sensitive detection of heroine based on surface- plasmonresonance has been theoretically and experimentally investigated. The experiment was realized by using an anti-MO monoclonal antibody and a morphine (MO)-bovine serum albumin (MOBSA) conjugate (antigen). The reason for using MO-BSA in the detection of heroine was also discussed. MO-BSA was immobilized on a gold thin film of SPR sensor chip by physical adsorption. The configuration of the device is allowed to be further miniaturized, which is required for the construction of a portable SPR device in the application of in-situ analysis.A simple technique for sensitive detection of heroine based on surface- plasmonresonance has been theoretically and experimentally investigated. The experiment was realized by using an anti-MO monoclonal antibody and a morphine (MO)-bovine serum albumin (MOBSA) conjugate (antigen). The reason for using MO-BSA in the detection of heroine was also discussed. MO-BSA was immobilized on a gold thin film of SPR sensor chip by physical adsorption. The configuration of the device is allowed to be further miniaturized, which is required for the construction of a portable SPR device in the application of in-situ analysis.

关 键 词：A Surface Plasmon Resonance-based Immunosensors for Sensitive Detection of Heroin SPW BSA 

分 类 号：R318.6[医药卫生—生物医学工程]