高甘油三酯血症对去卵巢大鼠股骨Fas/FasL表达的影响  

Effects of Hyper-triglyceride and Antilipemic Agents on Expressin of Fas/FasL in Femora of Ovariectomized Rats

作　　者：曾炼坤[1] 曾进龙[2] 邓煜辉[3] 刘雪芳[1] 夏碧文[1] 李万根[3] 

机构地区：[1]中山大学附属第二医院增城院区内分泌科,广州511300 [2]中山大学附属第二医院增城院区肿瘤血液科,广州511300 [3]广州医学院第二附属医院内分泌科,广州510260

出　　处：《中国医药导刊》2011年第10期1768-1769,1754,共3页Chinese Journal of Medicinal Guide

摘　　要：目的:探讨高甘油三酯(TG)血症对去卵巢大鼠股骨成骨细胞(OB)和破骨细胞(OC)中Fas、FasL的表达的影响。方法:将3月龄雌性SD大鼠40只,用果糖饲养复制高TG模型大鼠分为四组:(1)巢+果糖组;(2)去卵巢+果糖+非诺贝特(fenofibrate,FF)组;(3)去卵巢+普食组;④假手术+果糖组。12周后取股骨检测成骨细胞(OB)和破骨细胞(OC)中Fas、FasL的表达水平。结果:(1)去卵巢+果糖组的TG水平高于去卵巢+普食组(P<0.01),也高于去卵巢+果糖+FF组(P<0.01);(2)去卵巢+果糖组成骨细胞Fas、FasL的表达水平均高于去卵巢+果糖+FF组、去卵巢+普食组和假手术+果糖组(P<0.05);破骨细胞Fas、FasL的表达均低于其余三组(P<0.05)。结论:高TG血症使股骨组织中成骨细胞Fas、FasL表达增高,破骨细胞中Fas、FasL表达降低。Objective：To investigate hypertriglyceridemia and antilipemic agents on the expression of Fas, FasL in ovarieclomized rats.Methods：Forty female SD rats were randomly divided into 4 groups,（1）Ovariectomy ＋ fructose（OVX＋FO）,（2） Ovariectomy ＋ fructosc ＋fenofibrate（OVX＋FO＋FF）,（3）Ovariectomy ＋ normal diet（OVX ＋ND）,（4）Sham operation ＋ fructose（Sham＋FO）.Twelve weeks alter operation,all rats were sacrificed and right femora were taken.Then Fas,FasL expression were tested and analysed by pathological and immunohistochemical technology.Results：Hypertriglyceridemia down-regulated the expression of Fas,FasL on the osteoctasts but up-regulated Fas,FasL expression in osteoblasts;Fenofibrate up-regulated the expression of Fas,FasL expression in osteoclasts but down- regulated Fas,FasL expression in osteoblasts.Conclusions：Fructose can induce hypertriglyceridemia in ovariectomized rats which lead to down-regulate of Fas,FasL expression in osteoclasts and up-regulate Fas,FasL expression in osteoblasts.Hypertriglyceridemia may accelerate above changes and fenofibrate can prevent the adverse effect.

关 键 词：高甘油三酯血症 绝经后骨质疏松 FAS/FASL系统 成骨细胞 破骨细胞 

分 类 号：R589.2[医药卫生—内分泌]