甘露醇和当归对周围神经再灌注损伤的保护作用  

Comparisons of mannitol and danggui (Chinese angelica) for the protective effect on the injury of reperfusion in rabbit peripheral nerves

作　　者：王法[1] 黄耀添[1] 殷琦[1] 徐新智[1] 王军[1] 吕荣[1] 

机构地区：[1]中国人民解放军第四六三医院骨科,沈阳市110042

出　　处：《中华显微外科杂志》1999年第S1期39-41,共3页Chinese Journal of Microsurgery

摘　　要：目的　研究甘露醇和当归对周围神经再灌注损伤的保护作用。方法　观察神经电生理，神经内钙，自由基含量和组织结构的变化。　结果　缺血６ 小时应用甘露醇， 当归，再灌注５ 小时后潜伏期由（３７５６ ±０２０６） ｍ ｓ 减至（ ２２１６ ±００９６） ｍ ｓ 和（ ３４７２ ±０１６６ ） ｍ ｓ ， 传导速度由（１２４１８ ±１０１１）ｍ ／ｓ 增至（ ２１３２０ ±０６７６ ） ｍ ／ｓ 和（ １７１２０ ±１０３５ ） ｍ ／ｓ ， 波幅 由（ １２６２ ±０２５６ ） ｍ Ｖ 增 至（７１０８ ±０４３ ９） ｍ ｖ 和（２６０４ ±０２９ ７） ｍ Ｖ ， 神 经内钙 由（ ７７７９４ ±８ ２６８ ） μｇ／ｇ 减 至（ ４２１３８ ±３４８９） μｇ／ｇ 和（４００９４ ±２０８２） μｇ／ ｇ 。缺血６ 小时应用当归，甘露醇再灌３ ０ 分钟， 自由基由（３７５４５ ±４４７５） Ｙｍ ａｘ／μｇ 减至（２８００９ ±２１７６） Ｙｍ ａｘ／ μｇ 和（ １１１３６ ±１８ ０６） Ｙｍ ａｘ／ μｇ 。组织形态学的变化也说明损伤程度得到明显抑制。　结论　甘露醇和当归均可用于防治周围神经的再灌注损伤，Objective To compare the protection provided by danggui and mannitol against the injury incurred by ischemia and reperfusion in rabbit peripheral nerves Method The contents of calcium by spectroscopy atomic absorption and free radicals by electron spin resonance (ESR) spectroscopy We also made observations for electrophysiological and morphologic changes in rabbit peripheral nerves Results After 5h reperfusion with addition of 5% danggui at a dose of 1 mg/kg and 20% mannitol at a dose of 5 ml/kg respectively after 6h ischemia, Lan was reduced from (3 756±0 206)mS to (3 472±0 166)ms and (2 216±0 096)ms, NMCV increased from (12 418±1 011)m/s to (17 120±1 035)m/s and (21 320±0 676)m/s, Amp increased from (1 262±0 256)mV to (2 604±0 297)mV and (7 108±0 439)mV, the content of calcium reduced from (77 794±8 268)μg/g to (40 094±2 082)μg/g and (42 138±3 489)μg/g After 30 min reperfusion, the free radicals reduced from (37 545±4 475)Ymax/μg to (28 009±2 176)Ymax/μg and (11 136±1 806)Ymax/μg Our results also indicated that after administration of danggui or mannitol, the extents of tissue damage were inhibited The tion, it is very impor more potent than that of danggui Conclusion Both of the two drugs, danggui and mannitol, could be used to prevent and treat the reperfusion injury in rabbit peripheral nerves

关 键 词：周围神经 损伤 甘露醇 再灌注 

分 类 号：R965[医药卫生—药理学]