超氧化物歧化酶的荧光分析法  

Fluormentric Determination of Superoxide Dismutase

作　　者：林游宇[1] 郭祥群[1] 冯涛[2] 郑微云[2] 

机构地区：[1]厦门大学化学系,福建厦门361005 [2]厦门大学环境科学中心,福建厦门361005

出　　处：《福州大学学报（自然科学版）》1999年第S1期65-66,共2页Journal of Fuzhou University(Natural Science Edition)

基　　金：国家自然科学基金!(29677014)

摘　　要：对肾上腺素自氧化反应进行了研究， 发现反应中间产物能发出较强的荧光， 其生成量受超氧化物歧化酶的抑制． 据此建立了分析超氧化物歧化酶的新方法． 该方法线性范围为０ ～５２－６ｎｇ／ｍ Ｌ， 检测限为８－５６ｎｇ／ｍＬ， 相对标准偏差为１－６ ％ （ ｎ ＝ ６） ．A fluorescent method is proposed for the determination of superoxide dismutase (SOD) based on epinephrine autoxidation.We found that the intermediate product of the autoxidation process of epinephrine autoxidation.Can fluoresce rather strongly at 533 nm when excited at 333 nm. The amount of SOD is linearly dependent on the extent of the decrease of the fluorescence of the immediate.Under optimum conditions, the calibration graph was linear over a range of 0～53 ng/mL SOD.The detection limit was 8 6 ng/mL SOD.The relative deviation was 1 6% for 26 ng/mL SOD ( \%n\%=6 ).

关 键 词：超氧化物 歧化酶 荧光分析法 

分 类 号：Q503[生物学—生物化学]