耐尔蓝荧光探针法测定核酸  

Application of a Red-Region Fluorescence Dye,Nile Blue, in Nucleic Acids Assay

作　　者：陈秋影[1] 杨黄浩[1] 李东辉[1] 郑洪[1] 朱庆枝[1] 许金钩[1] 

机构地区：[1]厦门大学化学系,福建厦门361005

出　　处：《福州大学学报（自然科学版）》1999年第S1期105-106,共2页Journal of Fuzhou University(Natural Science Edition)

基　　金：国家自然科学基金!(29775021)

摘　　要：基于ＤＮＡ或ＲＮＡ对耐尔蓝荧光的猝灭效应， 建立一种新的ＤＮＡ 或ＲＮＡ 的测定方法． 在最优条件下， 测定小牛胸腺ＤＮＡ、鲑鱼精子ＤＮＡ、鲱鱼精子ＤＮＡ 和酵母ＲＮＡ 的线性范围分别为： ３－０ ～２－０ ×１０３ｎｇ／ｍＬ、９－０ ～２－０ ×１０３ｎｇ／ｍＬ、５－４ ～５－０ ×１０３ｎｇ／ｍＬ 和２７ ～１０ ×１０３ｎｇ／ｍＬ．检测限分别是３－０ｎｇ／ｍＬ、９－０ｎｇ／ｍＬ、５－４ｎｇ／ｍＬ和２７ｎｇ／ｍＬ， 相对标准差（ｎ ＝ ６） 是２－１ ％ ． 本文还讨论了干扰物质的影响．A novel fluorimetric method was proposed for the rapid determination of DNA and RNA based on their quenching effect on the cationic red-region fluorescent dye,nile blue.Under optimum conditions,the calibration curves for the determination of Calf thymus DNA(CT DNA),Salmon DNA(SM DNA),Herring sperm (HS DNA) and yeast RNA were linear over the range of 3 0-2 0×10 3 ng/mL,9 0-2 0×10 3 ng/mL,5 4-5 0×10 3 ng/mL and 27-10×10 3 ng/mL,respectively.The detection limits were 3 ng/mL for CT DNA,9 0 ng/mL for SM DNA,5 4 ng/mL for HS DNA and 27 ng/mL for RNA,respectively.The relative standard deviation(n=6) was within 2 1% in the middle of linear range. Interference concerning some interesting coexisting substances with the determination of DNA was also examined.

关 键 词：荧光探针 耐尔蓝 核酸 红区 

分 类 号：Q503[生物学—生物化学]