中华鲟组织培养的初步研究  

PRELIMINARY RESEARCH ON TISSUE CULTURE OF CHINESE STURGEON

作　　者：叶湘辉[1] 刘汉勤[1] 俞小牧[1] 张义兵[1] 常剑波[1] 

机构地区：[1]中国科学院水生生物研究所,武汉430072

出　　处：《水生生物学报》1999年第6期566-571,共6页Acta Hydrobiologica Sinica

基　　金：国务院三峡办;中国长江三峡工程开发总公司资助(三环科1-03-02-01)

摘　　要：取中华鲟的尾鳍、吻端、性腺等八种组织进行体外培养,得到来源于吻端和性腺组织的两个细胞株,分别命名为CSSn和CSG.CSSn和CSG均以成纤维样细胞为主。两种细胞都含有微染色体,染色体数目众多,在分布上具有“双峰多态”的特点,表明细胞已属异倍体。细胞生长较慢,CSG接种9d后、CSSn接种14d后数目达到最高值。CSG在16—30℃生长正常,最佳生长温度在27℃,最适pH为7.2。Cell strains were established from snout and gonad tissues of Chinese sturgeon, Acipenser Sinensis, among eight tissues for primary culture and designated CSSn and CSG, respectively. Biological characteristics were partly examined for CSSn and CSG. Both strains appeared fibroblastic. They had large quantities of chromosomes and possessed microchromo-somes. The distributions of their chromosome number exhibited the same feature described as 'two wave crests distinguished and a great variety of distribution' , which demonstrated that they became aneuploid. Growth curve of both strains showed their relatively slow replication. The highest cell number of CSG was observed after incubated 9 days, while CSSn, 14 days. The effects of temperature and pH on growth were examined for CSG. CSG remained a normal state at 16 - 30℃, with optimum temperature of 27℃, and pH condition of 7.2.

关 键 词：中华鲟 组织培养 染色体 微染色体 

分 类 号：Q959.4[生物学—动物学]