Quantitative in vitro assessment of drug phototoxicity by a chemiluminescence method  

作　　者：阎春林 廖康煌 胡跃 徐昱 

出　　处：《Chinese Medical Journal》1999年第6期21-23,共3页中华医学杂志（英文版）

摘　　要：Objective To establish a test model for phototoxic agents using the method of chemiluminescence Methods The phototoxicity of pipemidic acid, doxycycline, griseofuvin and chlorpromazine was detected These agents and distilled water were irradiated with ultraviolet A (UVA) in the presence of nicotinamide adenine dinucleotide reduced (NADH), and the duplicated samples were incubated in the dark as dark controls Then luminol was added to the test samples, and the chemiluminescent value was counted and calculated Results Chemiluminescent values of pipemidic acid, doxycycline and griseofuvin were significantly higher than those in controls The result of linear regression analysis showed that phototoxic intensity was linear correlated with UVA dosage The regression coefficient for distilled water was 0 56, indicating that the luminscent value (LV) rose slightly after UVA irradiation For pipemidic acid, griseofuvin and doxycycline, the regression coefficients reached 76 96 , 254 33 and 92 61 respectively, significantly increased in comparison with those of negative controls (P<0 01) Conclusion Phototoxicity of pipemidic acid, doxycycline and griseofuvin can be detected with the method of chemiluminescenceObjective To establish a test model for phototoxic agents using the method of chemiluminescence Methods The phototoxicity of pipemidic acid, doxycycline, griseofuvin and chlorpromazine was detected These agents and distilled water were irradiated with ultraviolet A (UVA) in the presence of nicotinamide adenine dinucleotide reduced (NADH), and the duplicated samples were incubated in the dark as dark controls Then luminol was added to the test samples, and the chemiluminescent value was counted and calculated Results Chemiluminescent values of pipemidic acid, doxycycline and griseofuvin were significantly higher than those in controls The result of linear regression analysis showed that phototoxic intensity was linear correlated with UVA dosage The regression coefficient for distilled water was 0 56, indicating that the luminscent value (LV) rose slightly after UVA irradiation For pipemidic acid, griseofuvin and doxycycline, the regression coefficients reached 76 96 , 254 33 and 92 61 respectively, significantly increased in comparison with those of negative controls (P<0 01) Conclusion Phototoxicity of pipemidic acid, doxycycline and griseofuvin can be detected with the method of chemiluminescence

关 键 词：ULTRAVIOLET CHEMILUMINESCENCE phototoxic agent 

分 类 号：R962[医药卫生—药理学]