L-NIL对脓毒血症模型小鼠细胞能量代谢及存活率的影响  

Effect of L NIL on cellular energy metabolism and survival rate in sepsis mice

作　　者：欧阳彬[1] 王吉甫[1] 

机构地区：[1]中山医科大学附属第一医院普通外科,广州510080

出　　处：《中华胃肠外科杂志》1999年第3期174-176,共3页Chinese Journal of Gastrointestinal Surgery

摘　　要：目的　观察诱导型一氧化氮合酶（ＮＯＳ） 抑制剂ＬＮ６亚氨乙基赖氨酸（ＬＮＩＬ）对脓毒血症小鼠细胞能量代谢和存活率的影响。方法　制作小鼠盲肠结扎穿孔（ＣＬＰ） 动物模型，分别注射０－９ ％ 氯化钠溶液或ＬＮＩＬ或ＬＮＩＬ加Ｌ精氨酸（ＬＡｒｇ） ，比较各组小鼠肝细胞线粒体结构、心肌ＡＴＰ、ＡＤＰ含量和小鼠存活情况。结果　ＬＮＩＬ使ＣＬＰ模型小鼠肝细胞线粒体结构损伤减轻，心肌细胞内ＡＴＰ含量增加，ＡＴＰ／ＡＤＰ 比例增加，小鼠１８ 小时存活率由２０％ 增加至６０ ％ 。结论　诱导型ＮＯＳ抑制剂ＬＮＩＬ能改善脓毒血症小鼠细胞能量代谢及提高其存活率。Objective To observe the effect of inducible nitric oxide synthase inhibitor L NIL (L N 6 iminoethyl lysine) on cellular energy metabolism and survival rate in cecum ligation puncture (CLP) model. Methods 0 9% natrii sodium or L NIL or L NIL + L Arg was injected into CLP mice respectively. The mitochondrial structure of liver cell, the content of ATP and the ratio of ATP/ADP of cardiomyocyte and the survival rate of mice were compared between groups. Results The damage of mitochondrial structure was attenuated, the content of ATP and the ratio of ATP/ADP of cardiomyocyte was increased,and the 18th hour survival rate raised from 20% to 60% in CLP mice with L NIL administration. Conclusion L NIL can improve the myocardium energy metabolism and raise the survival rate in CLP mice.

关 键 词：一氧化氮 脓毒血症 存活率 三磷酸腺苷 线粒体 

分 类 号：R392.3[医药卫生—免疫学]