Spectrofluorimetric Determination of Tetracycline Using Eu^(3+)Chelation and Cetyltrimethylammonium Bromide  

作　　者：Huang Zu-yun Chen Zhen-hua Yin Qu-an Shen Rong-sen 

出　　处：《Wuhan University Journal of Natural Sciences》1999年第2期103-107,共5页武汉大学学报（自然科学英文版）

摘　　要：The fluorescence of tetracycline was greatly enhanced by chelation with Eu 3+ in Tris HCl buffer (pH8.5). The addition of cetyltrimethylammonium bromide, a cationic surfactant, further enhanced the fluorescence of tetracycline Eu 3+ chelates.This spectrofluorimetric method for the determination of tetracycline yielded a linear responeses in the range of 10 nmol/L 10 μmol/L. The detection limit was 5 nmol/L (or 2.22 ng/mL).The recovery of tetracycline spiked in serum at levels of l and 0.09 μg/mL was 97.9% and 97.8%, respectively. This method is fast, sensitive and suitable for the determination of tetracycline in biological specimens.The fluorescence of tetracycline was greatly enhanced by chelation with Eu 3+ in Tris HCl buffer (pH8.5). The addition of cetyltrimethylammonium bromide, a cationic surfactant, further enhanced the fluorescence of tetracycline Eu 3+ chelates.This spectrofluorimetric method for the determination of tetracycline yielded a linear responeses in the range of 10 nmol/L 10 μmol/L. The detection limit was 5 nmol/L (or 2.22 ng/mL).The recovery of tetracycline spiked in serum at levels of l and 0.09 μg/mL was 97.9% and 97.8%, respectively. This method is fast, sensitive and suitable for the determination of tetracycline in biological specimens.

关 键 词：tetracycline (TC) SPECTROFLUORIMETRY cetyltrimethylammonium bromide (CTAB) 

分 类 号：R917[医药卫生—药物分析学]