Parathyroid Apoptosis and Proliferation in Uremic Patients with Severe Hyperparathyroidism  

作　　者：周富华 王笑云 

出　　处：《The Journal of Biomedical Research》1999年第1期12-16,共5页生物医学研究杂志（英文版）

摘　　要：Secondary hyperparathyroidism(SHPT) is characterized by an increase in parathyroid cell number and parathyroid hormone synthesis and secretion, but the importance in proliferation regulating of parathyroid cells is always ignored. In the present study, the expression of proliferation cell nuclear antigen (PCNA) and Bcl2(Bcell lymphoma/leukemia2) as well as the number of apoptotic cells in the diffuse and nodular parathyroid lesion of SHPT were evaluated, and compared with that in normal parathyroid glands. PCNA and Bcl2 had been measured by avidinbiotin complex methods, and apoptosis by in situ detection of nuclear DNA fragmentation. These results showed that the number of apoptotic cells was significantly decreased in SHPT compared to that in normal control; The appearance was more distinct in nodular parathyroid tissue than that in diffuse tissue of SHPT; The rate of PCNA positivecell was significantly increased in SHPT, and so was Bcl2. There was a negative correlation between apoptosis and PCNAapoptosis, as was between apoptosis and Bcl2 positive rate. These results suggested that the severe renal hyperparathyroidism may be mainly induced by excessive proliferation of parathyroid cells and reduced apoptotic process. Increased expression of Bcl2 may inhibit the parathyroid apoptosis and promote parathyroid proliferation in uremic patients.Secondary hyperparathyroidism(SHPT) is characterized by an increase in parathyroid cell number and parathyroid hormone synthesis and secretion, but the importance in proliferation regulating of parathyroid cells is always ignored. In the present study, the expression of proliferation cell nuclear antigen (PCNA) and Bcl2(Bcell lymphoma/leukemia2) as well as the number of apoptotic cells in the diffuse and nodular parathyroid lesion of SHPT were evaluated, and compared with that in normal parathyroid glands. PCNA and Bcl2 had been measured by avidinbiotin complex methods, and apoptosis by in situ detection of nuclear DNA fragmentation. These results showed that the number of apoptotic cells was significantly decreased in SHPT compared to that in normal control; The appearance was more distinct in nodular parathyroid tissue than that in diffuse tissue of SHPT; The rate of PCNA positivecell was significantly increased in SHPT, and so was Bcl2. There was a negative correlation between apoptosis and PCNAapoptosis, as was between apoptosis and Bcl2 positive rate. These results suggested that the severe renal hyperparathyroidism may be mainly induced by excessive proliferation of parathyroid cells and reduced apoptotic process. Increased expression of Bcl2 may inhibit the parathyroid apoptosis and promote parathyroid proliferation in uremic patients.

关 键 词：renal hyperparathyroidism proliferation cell nuclear antigen BCL2 

分 类 号：R692.502[医药卫生—泌尿科学]