微渗析取样技术与高效液相色谱-电化学检测用于对乙酰氨基酚药物动力学的研究  

High Performance Liquid Chromatography-Electrochemical Detection (HPLC-ECD)for the Pharmacokinetic Studies of Acetaminophen with Microdialysis

作　　者：施国跃[1] 徐芳[1] 金利通[1] 陆华[1] 

机构地区：[1]楚雄师专化学系

出　　处：《楚雄师范学院学报》1999年第3期71-75,共5页Journal of Chuxiong Normal University

摘　　要：本文以微渗析取样技术与高效液相色谱—电化学检测（HPLC—CED）相结合研究对乙酰氨基酚的药物动力学，流动相的磷酸盐缓冲溶液CPBS，PH＝7．07中，以四氛酞菁酮（CaTAPC）化学修饰电极作为工作电极，检测限为2．oXI0。VL，在6．0x10-7mol／L～1．0x10-4mol／L的线性浓度范围内，恒电位为＋500mV（对Agcl参比电极），对乙酰氢基酚与峰线电流呈良好的线性（有限好的电催化氧化性能），应用这个方法研究对乙酰氨基酚（ACE）的药物动力学，测得对乙酰氢酚与蛋白质结合参数（K）及结合因子（n）分别为5.37x103（mol／L）和257。测得（药物在血液中的）吸附半衰期为33．0±1．0min，消去半衰期为45．0±2．In this paper, we combined microdialysis sampling with HPLC - ECD and studied the pharmacokinetic of Acetaminophen. In the mobile phase of phosphate buffer solution (PBS, pH = 7.0), the Copper tetraaminophthalocyanine (CuTAPC ) chemically modified electrode , which was used as working electrode, has good electrocatalytical oxidation of Acetaminophen with a detection limit of 2 .0 x 10-7mol/L and a linear concentration range of 6.0 x 10-7mol/L - 1.0 x 10-4mol/L at + 500mV (vs.AgAgCl). .We applied this method to study the pharmacokinetic of Acetaminophen (ACE). The estimated association constant (K) and the number of the binding sites (n) on one molecule of BSA were 5.37 x 103 (mol/L) -1 and 2.57, respectively. The calculated half - life of absorption was 33.0 ±1.0 min, and that of elimination was 45.0±2. 0min.

关 键 词：高效液相色谱电化学检测 HPLC-ECD 药物动力学研究 对乙酰氨基酚微渗析 取析技术 

分 类 号：O657.1[理学—分析化学]