Y染色体探针示踪内皮祖细胞移植在损伤肺组织中的表达  

Appfication of Y chromosome specific probe to the detection of expression of endothelial progenitor cells grafted in the injured lung tissues of rats

作　　者：毛梅[1] 王博[2] 王艺[3] 徐剑铖[3] 傅祖红[1] 

机构地区：[1]广州军区武汉总医院呼吸内科,武汉430070 [2]郑州大学第一附属医院放射科 [3]第三军医大学新桥医院呼吸研究所

出　　处：《中华急诊医学杂志》2011年第11期1162-1166,共5页Chinese Journal of Emergency Medicine

摘　　要：目的探讨示踪损伤肺组织中移植内皮祖细胞（EPCs）的方法。方法将清洁级健康同遗传背景雌l生SD大鼠随机（随机数字法）分为3组（n=10）：PBS处理组、细胞移植组经尾静脉给予LPS建立急性肺损伤（ALI）模型，假损伤组给予等量PBS；30min后假损伤组和细胞移植组大鼠经静脉移植雄性大鼠EPCs（2×10^6／0．5mL），PBS处理组给予等量PBS；7d后取肺组织，采用RT—PCR和原位杂交（ISH）的方法检测Y染色体基因在损伤肺组织中的表达。组问比较采用ANOVA方差分析。结果细胞移植组肺内皮系统成功检测到Y染色体阳性信号，其它两组为阴性。结论Y染色体特异性探针可作为示踪干细胞移植的有效检测手段。Objective To investigate the efficient methods used for tracing endothelial progenitor cells （EPCs） after transplantation in injured lung tissue. Methods Thirty clean grade healthy syngeneic SD female rats were divided into three experimental groups （n = 10） ： （1） sham group, rats treated with intravenous phosphate-buffered saline （PBS） instead of lipopolysaccharide （LPS） followed by EPCs graft; （2） PBS-treated group, rats treated with intravenous PBS after intravenous injection of LPS to produce acute lung injury; （3） EPC-treated group, rats treated with EPCs after intravenous injection of LPS to produce acute lung injury. The transplanted EPCs were got from the same genetic species of the SD male rats. Rats of each group were sacrificed 7 days after EPCs transplantation. Their whole lung tissues were harvested to detect the expression of Y-chromosome by using hybridization in situ and RT-PCR assay. Statistic package of SPSS16. 0 was used for the data analysis and significant differences between means were evaluated by ANOVA analysis. Results Compared with the other two groups, positive signals of sex-determining region y were found in lung endothelium from the EPC-treated group. Conclusions Y chromosme specific probe can be one of efficient methods for tracing stem ceils after transplantation.

关 键 词：Y染色体 内皮祖细胞 干细胞移植 急性肺损伤 急性呼吸窘迫综合征 

分 类 号：R563[医药卫生—呼吸系统]