丁苯酞对脑低灌注老龄大鼠胆碱能神经功能的影响  

Effects of butylphthalide on the cholinergic nerve in cerebral hypoperfused old rats′ brains

作　　者：罗超[1] 苗亚玲[1] 赵万红[1] 龚坚[1] 李伏燕[2] 彭吉霞[1] 

机构地区：[1]湖北医药学院基础医学院,湖北十堰442000 [2]湖北医药学院附属太和医院

出　　处：《中国老年学杂志》2011年第21期4158-4160,共3页Chinese Journal of Gerontology

基　　金：湖北省自然科学基金项目(2009CDZ023);湖北医药学院博士启动金(2008QDJ6)

摘　　要：目的观察丁苯酞(NBP)对脑低灌注大鼠学习记忆的改善作用,探索其对胆碱乙酰基转移酶(ChAT)和乙酰胆碱酯酶(AChE)活力的影响。方法永久性结扎老龄大鼠双侧颈总动脉3个月,制备脑低灌注模型。实验分4组:假手术组、模型组、NBP 30和120 mg/kg组。后两组连续给予NBP45 d后,用Morris水迷宫检测大鼠的空间学习和记忆以及保存记忆的能力。同时,取皮层和海马,用生化法检测ChAT和AChE活力。结果水迷宫定位航行实验中,NBP可以显著缩短脑低灌注大鼠找到安全台的潜伏期。空间探索实验中,NBP明显增加脑低灌注大鼠在目标象限活动时间的百分比。同时,NBP可以显著增加脑低灌注大鼠皮层和海马ChAT活力,而对脑组织AChE的活力无明显影响。结论 NBP可以改善脑低灌注大鼠的学习记忆缺陷,其中通过增加了ChAT活力,改善胆碱能系统功能是其重要机制之一。Objective To observe the improving effects of butylphthalide（NBP） on the learning and memory of cerebral hypoperfused rats and investigate its effects on choline acetyltransferase（ChAT） and acetylcholine esterase（AChE） activity.Methods Cerebral hypoperfused models were made with ligating rats′ bilateral common carotid arteries for three months.There were four groups：sham,vehicle and NBP 30 and 120 mg/kg.The last two groups were administered NBP for 45 days consecutively.Then rats′ spatial learning and memory were measured with Morris water maze and the ChAT and AChE activity in rats′ brains were measured with biochemical method.Results In water maze positioning navigation trial,NBP reduced significantly the latencies of cerebral hypoperfused rats finding safe platform.In spatial exploring trial,NBP increased cerebral hypoperfused rats′ time percentages spent in the platform-quadrant.Meanwhile,NBP increased the ChAT activity in cerebral hypoperfused rats′cortex and hippocampus,but couldn′t impact rats′ brain AChE activity.Conclusions NBP could improve cerebral hypoperfused rats′ learning and memory deficits.Increasing the ChAT activity and amelioratingcholinergic system in brain is one of its important action mechanisms.

关 键 词：丁苯酞 学习记忆 胆碱乙酰基转移酶 乙酰胆碱酯酶 

分 类 号：R964[医药卫生—药理学]