银杏叶提取物对HBV相关肝癌形成过程的影响  

The effect of extract of ginkgo biloba leaf during the formation of HBV-related hepatocellular carcinoma

作　　者：王薇薇[1] 过建春[1] 荀运浩[1] 肖丽娜[1] 石伟珍[1] 施军平[1] 娄国强[2] 

机构地区：[1]杭州市第六人民医院中西医结合科,310014 [2]杭州师范大学附属医院

出　　处：《中华实验和临床病毒学杂志》2011年第5期325-327,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：基金项目：浙江省医药卫生科学研究基金（2007A164）;浙江省教育厅高校科研计划项目（20071092）;杭州市科技发展计划项目（2007Q04）

摘　　要：目的探讨银杏叶提取物对HBV相关肝癌形成过程的影响。方法99只HBV转基因小鼠随机分为对照组、高给药组、低给药组各33只，高和低给药组每天分别腹腔注射35mg／（kg·d）、17．5mg／（kg·d）的舒血宁注射液，对照组不做特殊处理。于12个月及18个月时进行检测。结果（1）HBV转基因小鼠在18个月可以发生肝癌，高和低给药组肝癌发生率低，与对照组差异有统计学意义。（2）高及低给药组12个月时肝脏HBx表达强度低于对照组，18个月时HBx、p53、Bcl-2表达强度低于对照组，差异均有统计学意义。（3）Spearman相关分析表明，肝癌的发生与肝组织HBx、p53、Bel-2表达呈一定程度正相关，r分别0．536、0．487、0．403，P均〈0．05。结论银杏叶提取物可使HBV转基因小鼠肝癌发生率显著降低，可能与其能一定程度降低HBV转基因小鼠肝脏HBx、p53、Bcl-2蛋白的表达水平有关。Objective To investigate the effect of extract of ginkgo biloba leaf（EGb） during the formation of HBV-related hepatocellular carcinoma （HCC）. Methods 99 HBV transgenic mice were randomly divided into control group, high-dose group, low-dose group. High-dose group and low-dose group were intraperitoneal injected 35mg/（kg @ d） and 17.5 mg/（ kg. d） of the shuxuening injection. Control group without special treatment. The serological markers and immunohistochemical markers in liver tissue will he done at the first 12 months and 18 months. Results （ i ） HBV transgenic mice can be found HCC at the 18 months. The incidence of HCC was lower in high-close group and low-dose group, there was statistically different among the three groups. （2） The semi-quantitative scoring of liver HBx expression was highest in the control group at the 12 months. The semi-quantitative scoring of liver HBx, p53 and Bcl-2 expression was highest in the control group at the 18 months. They all appeared statistically different among the three groups. （3） Spearman correlation analysis showed that HCC incidence and liver tissue HBx, p53, Bcl-2 expression was a certain degree of positive correlation, r was 0. 536, 0. 487 and 0. 403, P 〈 0. 05. Conclusion EGb can reduced the incidence of the HCC with HBV transgenie mice. The reason may be that the EGb can reduce liver HBx, p53, Bcl-2 protein expression in the HBV transgenic mice.

关 键 词：二裂银杏 肝炎病毒 乙型 癌基因 基因转变 肝肿瘤 基因 p53 

分 类 号：R285[医药卫生—中药学]