无锡市流感病毒抗体水平调查  

A survey of levels of antibodies against influenza viruses in the population of Wuxi City

作　　者：尤凤兴[1] 孙纳[1] 马广源[1] 王苗苗[1] 沙丹[1] 施超[1] 吴家林[1] 凌霞[1] 肖勇[1] 钱燕华[1] 姜永[1] 谢洁[1] 季亚勇[1] 管红霞[1] 

机构地区：[1]无锡市疾病预防控制中心,214023

出　　处：《中华实验和临床病毒学杂志》2011年第5期351-354,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：基金项目：江苏省卫生科研项目（H200956）;江苏省预防医学科研项目（Y201026）;无锡市科技发展计划（CSZ00955,CSE009012,CSz01051）

摘　　要：目的调查无锡市人群中甲型、乙型流感病毒抗体水平和新甲型H1N1流感病毒传人前后人群中抗体水平，并对新甲型H1N1流感病毒传人1年后自然人群中成人抗体水平与接种新甲型H1N1流感疫苗后1年的成人抗体水平进行比较。方法收集2008年9月至2009年5月、2010年9月至2011年1月无锡市不同年龄段人群血清和接种新甲型HIN1流感疫苗1年的成人血清，用血凝抑制（HI）试验测定抗体，并比较不同时间段各人群中的流感抗体阳性率、保护率和几何平均滴度（GMT）。结果新甲型H1N1流感病毒传入前，无锡市自然人群的HI抗体阳性率为2．86％（4／140），保护率为0．71％（1／140），GMT为5．23。新甲型H1N1流感病毒传入1年后，自然人群的HI抗体阳性率为66．33％，保护率为37．76％、GMT为19．17；其中成人HI抗体阳性率、保护率和GMT分别为50．00％、19．44％和13．09。接种新甲型H1N1流感疫苗的成人1年后HI抗体阳性率、保护率和GMT分别为61．36％、22．73％和14．14，与自然人群中成人在流感病毒传入1年后的抗体水平差异无统计学意义（P均〉0．05）。无锡市人群中甲型与乙型流感病毒HI抗体水平分别为：H1N1病毒抗体阳性率为55．00％，保护率为35．00％，GMT16．90；H3N2抗体阳性率为86．40％，保护率为84．30％，GMT为58．56。结论新甲型H1N1流感病毒传入无锡市1年后，自然人群中新甲型H1N1流感病毒抗体阳性率、保护率和GMT均已达到季节性流感抗体水平。同时人群中已有一定水平的甲型、乙型流感病毒抗体，近期不会发生较大的季节性流感疫情。Objective To investigate levels of antibodies against type A and type C influenza viruses and those against the 2009 H1N1 influenza A virus （before and after the 2009 H1N1 pandemic） among residents in Wuxi. To compare levels of antibodies against the 2009 HI N1 influenza virus （one year after the pandemic） in the unvaccinated population with those in the population who received vaccine. Methods Serum samples were collected from subjects （aged 1-60 years） during September 2008 to May 2009, and during September 2010 to January 2011. Also collected were serum samples from adults who had received vaccines for pandemic （H1N1） 2009 for one year. Antibody response to influenza viruses was measured using hemagglutination inhibition （HI） assay. Seropositivity rate, seroprotection rate and geometric mean titer （GMT） were compared for each age group during different periods. Results Before the outbreak of the 2009 H1N1 pandemic, seropositivity rate, seroprotection rate and GMT among the study subjects in were 2. 86% （4/140） , 0. 71% （1/140） and 5.23, respectively. One year after the outbreak, seropositivity rate, seroprotection rate and GMT among the study subjects were 66.33% , 37.76% and 19.17, respectively. Among them, adult subjects showed 50.00% seropositivity rate, 19.44% seroprotection rate and 13.09 GMT, while adult subjects who had received vaccine for one year showed 61.36% seropositivity rate, 22.73% seroprotection rate and 14. 14 GMT. No significant difference wasobserved between these two populations （P 〉 0.05 for all three indexes）. Furthermore, before the outbreak of the 2009 H1N1 pandemic, levels of antibodies against seasonal influenza viruses among the study subjects were as follows： for H1N1 virus, seropositivity rate, seroprotection rate and GMT were 55.00% , 35.00% and 16.90, respectively; for H3N2 virus, seropositivity rate, seroprotection rate and GMT were 86.40% , 84.30% and 58.56, respectively. Conclusion One year after the 2009 H1N1 influenza A virus had spre

关 键 词：流感病毒A型 中和试验 

分 类 号：R51[医药卫生—内科学]