HFRS患者尿中病毒抗原检测的实验研究  

Experimental study to detect HFRSV antigen in urine from HFRS Patients

作　　者：张建傲[1] 罗端德[1] 李淑莉[1] 曾令兰[1] 罗全忠 冷世信 唐天侯 徐新献 

机构地区：[1]同济医科大学附属协和医院传染科,武汉430022 [2]湖北省宜城市医院 [3]湖北省咸宁地区医院 [4]湖北省咸宁市医院 [5]湖北省通城县医院

出　　处：《中华疾病控制杂志》1998年第3期177-178,共2页Chinese Journal of Disease Control & Prevention

摘　　要：目的建立和评价IEDA检测HFRS患者尿中病毒抗原的方法及敏感性和特异性。方法采用自行制备的免抗HFRSV多克隆抗体（Anti－HFRSVIgG），运用免疫酶斑点法（IEDA）检测40例HFRS患者尿中的病毒抗原，同时采用传统的间接免疫荧光法（I－IFA）检测患者血清中抗流行性出血热病毒抗原的IgM型抗体（Anti－HFRSVIgM）作相关分析。结果应用IEDA检测尿中HFRS抗原的总阳性率为65％，早期（5日内）抗原检出率为83．33％，而应用I－IFA检测血清中Anti－HFRSVIgM阳性率为77．8％。结论IEDA与I－IFA检测的结果高度相关，且5病日内的早期诊断率为前者高于后者。Objective To establish the method by using immune enzyme dot assay (IEDA) to detect HFRSV antigen, and to evaluate the sensitivity and speificity Of this method. Methods Animmune enzyme dot assay(IEDA) was employed to detect HFRSV antigen in urine from HFRS patients, and compared with indirect immune nurorescence assay (I-IFA), 4o urine sample were detected in this study. Results Total positive rate of HFRSV-antigen detected by IEDA was 65% andpositive rate in primary phase (within 5 days) of HFRSV-antigen was 83. 33% in urine, while thatof anti-HFRSVIgM detected by I-IFA was 77. 8% in serum. Correlation study was employed, thatshowed a highly correlation in the results of IEDA and I-IFA. ConcluSions As compared with IIFA, IEDA is a more specific, sensitive, rapid and simple method with higher POSitive rate in primary phase, lower cost and without special equipment and could be widely used for early diagnosis ofHFRS in hospital at basic levels.[

关 键 词：肾综合征出血热 肾综合征出血热病毒 免疫酶斑点法 

分 类 号：R446.6[医药卫生—诊断学]