Phenomenon and mechanism of "secondary damage" during repair of DNA damage in irradiated mammalian cells  

作　　者：Luo Ying Sun Zhixian Wu Zuze 

机构地区：[1]Inst Radiat Med, Beijing 1000850, Peoples R China

出　　处：《Chinese Science Bulletin》1998年第11期921-925,共5页

基　　金：ThisworkwassupportedbytheNationalNaturalScienceFoundationofChina (GrantNo .39470 799)

摘　　要：By means of comet assay, a study of kinetics curve of DNA damage repair in irradiated SX-9 cells that came from mouse breast cancer proceeded. It was found that while the initial DNA damages had nearly been repaired, DNA damages arose for the second time, then they were repaired again. As a result, a phenomenon of "secondary damage" was found during the repair of DNA damages in irradiated SX-9 cells. Further research illuminated that 3-aminobenzamide (3AB), which is an inhibitor of poly (ADP-ribose) polymerase, could change the proceeding of "secondary damage". For this reason it is possible that there exists some inner relationship between the phenomenon of "secondary damage" and the function of poly(ADP-ribose) polymerase.By means of comet assay, a study of kinetics curve of DNA damage repair in irradiated SX-9 cells that came from mouse breast cancer proceeded. It was found that while the initial DNA damages had nearly been repaired, DNA damages arose for the second time, then they were repaired again. As a result, a phenomenon of “secondary damage” was found during the repair of DNA damages in irradiated SX-9 cells. Further research illuminated that 3-aminobenzamide (3AB), which is an inhibitor of poly (ADP-ribose) polymerase, could change the proceeding of “secondary damage”. For this reason it is poasible that there exists some inner relationship between the phenomenon of “secondary damage” and the function of poly(ADP-ribose) polymerase.

关 键 词：DNA DAMAGE REPAIR 'secondary damage' poly(ADP-ribose) polymerase. 

分 类 号：Q691[生物学—生物物理学]