脂联素水平及基因多态性与代谢综合征的关系  

Relationship of the adiponectin level and gene polymorphism with the metabolic syndrome

作　　者：陈芳[1] 苏颖[1] 李晓霞[1] 武海亮[2] 王洁[3] 杨锐英[2] 

机构地区：[1]宁夏医学院第二附属医院银川市第一人民医院,银川750001 [2]宁夏医科大学附属医院心脏中心干部病房 [3]宁夏医科大学医学科学技术研究中心

出　　处：《临床心血管病杂志》2011年第11期845-848,共4页Journal of Clinical Cardiology

基　　金：宁夏自然科学基金项目(No:NZ09109)

摘　　要：目的:探讨脂联素(APN)水平及其单核苷酸基因多态性(SNP)+45T/G和+276G/T两个位点与代谢综合征(MS)的关系。方法:238例研究对象分为对照组和MS组,采用实验-对照研究方法,应用酶联免疫吸附法(ELISA)及聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,对血浆APN浓度及其2个位点基因多态性进行检测。结果:与对照组比较,MS组APN水平降低(P<0.05);MS组SNP+276T等位基因分布明显升高,多因素逐步Logistic回归分析显示SNP+276基因多态性为MS危险因素。结论:MS患者APN水平下降,携带SNP+276T等位基因者患MS的风险增加。Objective：Researching the relationship of the adiponectin（APN） level,single nucleotide polymorphism（SNP） ＋45 T/G and ＋276 G/T with the metabolic syndrome（MS）.Method：The total of 238 patients were divided into normal control group and MS group.Adopting experimental-control study,applying the technique of enzyme-linked immunosorbent assay（ELISA） and polymerase chain reaction-restriction fragment length polymorphism（PCR-RELP） to detect concentration of the plasma adiponection and polymorphisms of the two polymorphisms of adiponectin.Result：Comparing the two groups,APN of the MS group reduced（P0.05）,the genotype frequency of MS group on SNP＋276T alleles goes up obviously.Proceeding multiple factors gradually logistic,regression analysis shows that SNP＋276 locus are risk factors to effect MS.Conclusion：APN level of patients with MS decline;People who carries the SNP＋276T alleles would run more risk on affecting MS.

关 键 词：脂联素 代谢综合征 基因多态性 

分 类 号：R344[医药卫生—基础医学]