美托洛尔对红细胞血红蛋白携氧能力的影响  

Effect of Metoprolol on the oxygen carrying ability of hemoglobin

作　　者：单光华[1] 李自成[1] 黄耀熊[2] 吴正洁[2] 郭军[1] 

机构地区：[1]暨南大学附属第一医院心内科,广州510630 [2]暨南大学生物医学工程研究所

出　　处：《临床心血管病杂志》2011年第11期855-857,共3页Journal of Clinical Cardiology

基　　金：广东省医学科研基金资助(No:A2008357);暨南大学第一临床医学院重点学科基金资助(No:2010-4)

摘　　要：目的:探讨美托洛尔是否影响红细胞血红蛋白的携氧能力。方法:采集正常人红细胞,悬浮于不同pH值(pH7.4、pH5.0)等渗PBS缓冲液,分别用肾上腺素、美托洛尔、美托洛尔+肾上腺素预处理红细胞,在拉曼光谱点扫描分析检测单个活态红细胞内血红蛋白特征光谱变化。结果:在pH5.0时,氧化血红蛋白特征峰峰强在PBS、美托洛尔的作用下明显降低;在肾上腺素的作用下显著升高;而经美托洛尔+肾上腺素预处理后,肾上腺素的这一作用不明显。结论:美托洛尔能减轻肾上腺素在酸性环境中增加红细胞血红蛋白的氧合力的作用,增加氧从血红蛋白的解离,有利于缺氧组织的供氧。Objective：To investigate the effect of Metropolol on the oxygen carrying ability of hemoglobin.Method：Erythrocytes from healthy people were collected,suspended in different pH value（pH7.4,pH5.0） of isotonic buffer and pretreated with adrenaline,Metropolol,adrenaline＋Metropolol respectively.The characteristic spectral changes of hemoglobin in single living erythrocyte were detected with Raman spectroscopy analysis.Result：①Characteristic peak of oxyhemoglobin in erythrocyte was significantly improved in acid environment（P0.05）.②While no significant change of characteristic peak was observed in pretreatment of Metropolol alone whether it is in acid environment or not,which is similar to PBS treatment group.③Characteristic peak of oxyhemoglobin in erythrocyte was significantly improved by the treatment of adrenaline in acid environment（P0.05）.④While the similar effect of adrenaline can not be observed after the pretreatment of Metropolol（P0.05）.Conclusion：Metropolol pretreatment decreases the effect that adrenaline can enhance the oxygen carrying ability of hemoglobin in acid environment,and enhances the dissociation of oxygen from hemoglobin,supplying more oxygen to oxygen-starved tissue.

关 键 词：红细胞 血红蛋白 光谱 

分 类 号：R331.1[医药卫生—人体生理学]