Transduction of human hepatocellular carcinoma cells with human γ-interferon gene via retroviral vector  

作　　者：QIAN Shu Bing and CHEN Shi Shu 

出　　处：《World Journal of Gastroenterology》1998年第3期30-33,共4页世界胃肠病学杂志（英文版）

摘　　要：AIM To investigate the therapeutic potential of gamma interferon (IFN γ) gene modified human hepatocellular carcinoma (HCC) cells. METHODS The IFN γ gene was introduced retrovirally into four HCC cell lines. Secreted IFN γ activity was assessed using bioassay. The expression of MHC molecules was detected by FACS. Tumorigenicity was analysed by tumor formation in nude mice. RESULTS Four IFN γ gene transduced HCC cell lines secreted different amounts of IFN γ, as in the same case of five clones derived from one HCC cell line. Transduction with IFN γ caused significant increase in the expression of major histocompatibility complex (MHC) antigens on HCC cells. The expression of HLA class Ⅰ was increased by 2-3 times in terms of mean fluorescence intensities, while for class Ⅱ expression, the percentage of positive cells augmented from <10% to >50%. When equal amount of tumor cells were injected into nude mice, the tumorigenicity of some transduced cells decreased dramatically. CONCLUSION IFN γ gene transduction can convert weakly immunogenic HCC cells to activate antitumor immune response, and further pave the way for the future use of such gene modified tumor cells as a modality for the cancer immunotherapy.

关 键 词：carcinoma hepatocellular/therapy INTERFERON Ⅱ/genetics RETROVIRIDAE immunotherapy adoptive 

分 类 号：R735.7[医药卫生—肿瘤]