Lef-1基因在棕色和白色羊驼皮肤差异表达  

Differential Expression of Lef-1 Gene in Brown and White Alpaca Skin

作　　者：杨磊[1] 王海东[1] 王祎[2] 于秀菊[1] 董彦君[1] 董常生[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《中国生物化学与分子生物学报》2011年第11期1061-1066,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：山西省科技攻关项目(No.2006031060);山西农业大学创新基金(No.2009001)~~

摘　　要：本实验旨在研究淋巴增强因子-1(Lef-1)在不同毛色羊驼皮肤中的表达和定位,探索其与毛色间的关系.采用实时荧光定量PCR、Western印迹以及免疫组织化学方法,研究白色和棕色羊驼皮肤中的mRNA、蛋白表达水平和定位.定量实时荧光PCR结果显示,Lef-1在棕色羊驼皮肤组织相对基因表达量是3.372 7±0.198 9,在白色羊驼皮肤组织是1.000 3±0.022 7;Western印迹结果显示,在羊驼皮肤组织总蛋白中存在分子量约44 kD与兔抗Lef-1多克隆抗体发生免疫阳性反应的蛋白条带,棕色羊驼平均蛋白表达量显著高于白色羊驼;免疫组织化学结果显示,在棕色羊驼皮肤组织中多表达在毛球部,表皮也有分布,在白色羊驼皮肤组织中多表达在表皮,在棕色和白色羊驼皮肤组织中外根鞘都有较强表达.结果显示,Lef-1在棕色和白色羊驼皮肤的定位和含量存在差异,提示Lef-1可能影响了羊驼被毛颜色的形成.To study the expression and localization of lymphoid enhancer factor-1（Lef-1） in alpaca skin of different colors in the process of coat color formation,the mRNA and protein expression levels of Lef-1 were examined by quantitative real-time PCR（QRT-PCR）,Western blotting and immunohistochemistry.The gene expressive quantity of Lef-1 in the brown alpaca skin was 3.372 7±0.198 9,and that in white alpaca skin was 1.000 3±0.022 7.The average protein expression of Lef-1 in brown alpaca skin was significantly higher than that in white alpaca.The molecular weight of Lef-1 protein was about 44 kD.The distribution of Lef-1 was different between the white and brown alpaca skin.The results showed that Lef-1 may involve in the coat color formation in alpaca.

关 键 词：淋巴增强因子-1(Lef-1) 羊驼 定量实时荧光PCR 蛋白质印迹 免疫组织化学 

分 类 号：Q78[生物学—分子生物学]