RT-PCR法检测HGV RNA和ELISA法检测抗-HGV意义探讨  

SIGNIFICANCE OF DETECTING HGV RNA BY RT- PCR AND ANTI - HGV BY ELLSA

作　　者：陈雪娟[1] 李刚[1] 陈文思[1] 刘淑芳[1] 黄仰苏 

机构地区：[1]中山医科大学附属三院传染科,广州510630

出　　处：《实用肝脏病杂志》1998年第1期5-6,共2页Journal of Practical Hepatology

基　　金：国家自然科学基金(39600130);广东省卫生厅科研基金

摘　　要：目的:探讨检测HGV RNA和抗-HGV的临床意义。方法:采用逆转录聚合酶链反应(RT-PCR)技术和ELISA法分别对351例住院肝炎患者血清中HGV RNA和抗-HGV进行检测。结果:351例中有32例(9.11％)HGV RNA阳性,34例(9.68％)抗HGV阳性,5例(1.41％)两项同时阳性;在11例患者不同住院期间的检测中发现,7例检测结果无变化,1例HGV RNA阴转,1例抗-HGV阴转,2例抗-HGV阳转。结论:HGV RNA和抗-HGV同时阳性的病例比较少。大多数情况两者不是同时存在,诊断意义可能有所差异;HGV RNA阳性表示现症感染,而抗-HGV阳性可能是感染后期或恢复期的标志。在HGV感染过程中会出现HGV RNA阴转或抗-HGV阴转和阳转的可能。Objective To explore the clinical significance of detection of hepatitis G virus (HGV) RNA and anti-HGV.Methods HGV RNA and anti-HGV were detected in 351 patients with hepatitis by RT-PCR and ELISA,respectively.Results 32 of 351 cases (9.11%) were positive for HGV RNA and 34 positive for anti-HGV.HGV RNA and anti-HGV coexisted in 5 sera.Serum HGV RNA or anti-HGV didn't change in 7 of 11 patients observed during the hospitalization Periods, while 1 turned to HGV RNA negative, 1 to anti-HGV negative and 2 to anti-HGV positive.Conclusions HGV RNA and anti-HGV did not coexist in most cases and might have different significance in diagnosis of HGV infection.HGV RNA suggestes present infection, While anti-HGV may indicate postin-fection.During the course of HGV infection, serum HGV RNA and anti-HGV are changeable.

关 键 词：庚型肝炎病毒 聚合酶链反应 

分 类 号：R446.61[医药卫生—诊断学]