Mechanisms of peroxynitrite-induced [Ca^(2+)]_i increase in single neuronal cell  

作　　者：YI Yong CAI Dong GAO Zhonghong YANG Xiangliang QU Anlian XU Huibi WANG Xiaomin HAN Songping 

机构地区：[1]Institute of Biophysics and Biochemistry,Huazhong University of Science and Technology,Wuhan 430074,China [2]Neurosscience Research Centes Beijing Medical University,Beijing 100083.China [3]Department of Phammacol and Phyaiol Sci.St Louis Univ School of Mec St Louis MO 63104,USA.

出　　处：《Chinese Science Bulletin》1998年第20期1727-1732,共6页

摘　　要：The mechanism of peroxynitrite (ONOO -)induced [Ca 2+ ] i increase in single MN9D cell (Dopaminergic neuroblastoma cell line) was studied by using Fura2 microfluorometric technique. The results show that ONOO - caused a rapid increase of [Ca 2+ ] i when ONOO - was puffed to the cell. Removing Ca 2+ from the bath or using calcium channel antagonist (CdCl 2, Nifedipine) greatly inhibited the [Ca 2+ ] i increase induced by ONOO -, suggesting that the opening of LCa 2+ channel makes a great contribution to the [Ca 2+ ]\-i increase. The effect of sulfhydryl reductive agent (DTT) on ONOO -induced [Ca 2+ ]\-i increase suggests that ONOO -activating LCa 2+ channel is partly related to its oxidative speciality.The mechanism of peroxynitrite (ONOO -)_induced [Ca 2+ ] i increase in single MN9D cell (Dopaminergic neuroblastoma cell line) was studied by using Fura_2 microfluorometric technique. The results show that ONOO - caused a rapid increase of [Ca 2+ ] i when ONOO - was puffed to the cell. Removing Ca 2+ from the bath or using calcium channel antagonist (CdCl 2, Nifedipine) greatly inhibited the [Ca 2+ ] i increase induced by ONOO -, suggesting that the opening of L_Ca 2+ channel makes a great contribution to the [Ca 2+ ]\-i increase. The effect of sulfhydryl reductive agent (DTT) on ONOO -_induced [Ca 2+ ]\-i increase suggests that ONOO -_activating L_Ca 2+ channel is partly related to its oxidative speciality.

关 键 词：peroxynitrite(ONOO-) intracellular calcium concentration([Ca2+]i) MN9D cells. 

分 类 号：Q25[生物学—细胞生物学]