Germination of embryo in soybean anther culture  

作　　者：Guilan Zhao Yanzhi Liu Aiping Yin Junbo Li 

机构地区：[1]Jilin Acad Agr Sci, Prov Key Open Lab Biotechnol, Gongzhuling 136100, Peoples R China

出　　处：《Chinese Science Bulletin》1998年第23期1991-1995,共5页

摘　　要：A large number of experiments about induction of callus, embryo and germination of regeneration plants were done in soybean anther culture. Screening for genotypes, low_temperature pretreatment for floral buds, constituent of hormone in medium and effects of different temperatures on induction rate of callus and embryo were studied. It was found that the TDZ played an important role in embryo induction and germination. 28 genotypes had reaction competent to the anther culture in all 110 genotypes. Induction rate of callus was 26%-68.1%. Plant differentiation rate was 0.5%-2.0%.A large number of experiments about induction of callus, embryo and germination of regeneration plants were done in soybean anther culture. Screening for genotypes, low-temperature pretreatment for floral buds, constituent of hormone in medium and effects of different temperatures on induction rate of callus and embryo were studied. It was found that the TDZ played an important role in embryo induction and germination. 28 genotypes had reaction competent to the anther culture in all 110 genotypes. Induction rate of callus was 26%–68.1%. Plant differentiation rate was 0.5%–2.0 %.

关 键 词：GLYCINE max\% L. ANTHER culture EMBRYO germination. 

分 类 号：S565.1[农业科学—作物学] Q944.4[生物学—植物学]