一期凝固法测定尿液组织因子促凝活性的方法学建立  

Analysis of urinary tissue factor procoagulant activity

作　　者：付文金[1,2] 郑磊[1] 邓任堂[2] 陈楚甜[2] 叶长钦[2] 王少波[2] 刘亮[2] 陈梅莲[2] 彭兰芬[2] 熊石龙[1] 包杰[1] 汤惠华[2] 王前[1] 

机构地区：[1]南方医科大学南方医院,广东广州510282 [2]东莞市厚街医院,广东东莞523945

出　　处：《热带医学杂志》2011年第10期1134-1137,共4页Journal of Tropical Medicine

基　　金：东莞市重点课题项目(200910515000104)

摘　　要：目的建立一种简便检测尿液组织因子促凝血活性的方法。方法以owren-koller稀释液5倍稀释尿液后,在STAGO全自动凝血仪检测凝固时间,通过兔脑粉不同浓度及其对应的凝固时间确定相应的标准曲线,计算出尿液组织因子(TF)的促凝血活性(TF-PCA),并对该方法进行评价。结果通过owren-koller稀释液处理尿液有效解决了尿液理化因素变异大、干扰物质多等对TF活性检测的影响,TF中和单抗可有效抑制PCA活性,说明尿液PCA活性源于TF。TF-PCA测定方法学评价:总不精密度分别小于7%,表明该方法重复性尚可,平均回收率为80.8%,最低检测限为5mu/L,并在15～2200mu/L之间线性良好。结论一期凝固法是一种简便、结果可靠的检测尿液TF-PCA的方法。Objective To establish a simple method to analyze the urinary tissue factor procoagulant activity（TF-PCA）. Methods Urine was diluted with owren-koller and then urinary TF-PCA was analyzed by one-stage coagulantation with STAGO auto-analyzer.The standard curve between PCA and coagulation time was determined by the different concentration of TF.The method evaluation was conducted. Results Neutralizing monoclonal antibody of TF could effectively inhibit the PCA of urine, so it could verify the PCA originated from TF. Dilution of urine with owren-koller could avoid the effects of variation of urine on TF analysis. Urinary TF-PCA which was measured with a total imprecison less than 7% and a recovery rate of 80.8%, meeting the analytical goal.The lower limit of detection was 5 mu/L,and a good linear relationship was observed when the concentration range is from 15 to 2 200 mu/L. Conclusion One-stage coagulantation was a simple and reliable method to analyze the urinary TF-PCA.

关 键 词：一期凝固法 尿液 组织因子促凝血活性 

分 类 号：R446.12[医药卫生—诊断学]