Determination　of　Amino　Acidsas　Their　N-Hydroxy-succinimidyl-3-indolylacetate　Derivatives　by　Reversed-phase　HPLC  

作　　者：QIU Pei hong (Department of Chemistry, Wenzhou Medical College, Wenzhou, 325003) ZHANG Hua shan, XU Guo liang and CHENG Jie ke (Department of chemistry, Wuhan University, Wuhan, 430072) 

出　　处：《Chemical Research in Chinese Universities》1997年第1期16-22,共7页高等学校化学研究（英文版）

摘　　要：The present paper covers the reversed phase HPLC separation and determination of fourteen derivatized amino acids with a new reagent N hydroxy succinimidyl 3 indolylacetate(SIIA). The derivatives of the amino acids were eluted by a mobile phase of methanol water containing 10 mmol/L H 3cit Na 2HPO 4 buffer of pH 3 5 and 1 2 mmol/L tetrabutylammonium bromide on an ODS column. The detection limits were pmol level or so, respectively, when a signal to noise ratio was 3. The method was applied to the determination of amino acids in tea sample with satisfactory results.The present paper covers the reversed phase HPLC separation and determination of fourteen derivatized amino acids with a new reagent N hydroxy succinimidyl 3 indolylacetate(SIIA). The derivatives of the amino acids were eluted by a mobile phase of methanol water containing 10 mmol/L H 3cit Na 2HPO 4 buffer of pH 3 5 and 1 2 mmol/L tetrabutylammonium bromide on an ODS column. The detection limits were pmol level or so, respectively, when a signal to noise ratio was 3. The method was applied to the determination of amino acids in tea sample with satisfactory results.

关 键 词：Liquid chromatography N    Hydroxy  succinimidyl  3  indolylacetate Amino acid 

分 类 号：O621[理学—有机化学]