Studies　on　the　Binding　of　Tris(1,10-phenanthroline)　Nickel(Ⅱ)　to　Calf　Thymus　DNA  

作　　者：JIN Lan , YANG Pin . . and LI Qing-shan (State Key Laboratory of Coordination Chemistry, Nanjing University , Nnjing 21 0093 ) YANG Bin-sheng (Institute of Molecular Science , Shanxi University , Taiyuan , 030006 ) 

出　　处：《Chemical Research in Chinese Universities》1997年第3期201-206,共6页高等学校化学研究（英文版）

摘　　要：Absorbance and fluorescence methods were used to study the interaction of - and A-tris (phenanthroline) nickel ( Ⅱ) complex with calf thymus DNA. It was con- cluded that -Ni(phen). preferentially bound to DNA. Especially the fluorescence Scatchard plots were discussed for the binding of EthBr to calf thymus DNA in the presence of varying concentrations of the metal complex. Theses studies indicated that both the isomers bound to DNA by two acting types , namely , intercalative and electrostatic bound modes.Absorbance and fluorescence methods were used to study the interaction of - and A-tris (phenanthroline) nickel ( Ⅱ) complex with calf thymus DNA. It was con- cluded that -Ni(phen). preferentially bound to DNA. Especially the fluorescence Scatchard plots were discussed for the binding of EthBr to calf thymus DNA in the presence of varying concentrations of the metal complex. Theses studies indicated that both the isomers bound to DNA by two acting types , namely , intercalative and electrostatic bound modes.

关 键 词：Chiral metal complexe DNA . Binding mode 

分 类 号：O614.813[理学—无机化学]