Significance　of　P-selectin　expression　in　human　glomerulonephritis  

作　　者：李晓 周同 郝翠兰 董德长 程砜 陈诗书 

出　　处：《Chinese Medical Journal》1997年第7期16-18,共3页中华医学杂志（英文版）

摘　　要：Objective To examine the relationship between P selectin expression and human glomerulonephritis. Methods P selectin expression was investigated in renal biopsies of glomerulonephritis patients (n=133) by immunohistochemical and in situ hybridization analyses. They were divided into three groups based on the degrees of cells proliferation or sclerosis. Group Ⅰ: histological lesions in glomeruli are mild (n=23); Group Ⅱ: glomerular cells proliferation is prominent (n=91); Group Ⅲ: glomerular sclerosis is severe (n=19). The Kruskal Wallis test, Chisquare test and Spearman's correlation were used in our study. Results In normal controls, renal P selectin expression was negative (n=10). In glomerulonephritis, the up regulated P selectin expression on tubular epithelium was significantly higher than that on glomeruli and interstitium (P<0.01). P selectin was expressed predominantly on platelets in glomeruli, and glomerular P selectin expression was more significantly up regulated in Group Ⅱ than in Group Ⅰ or Group Ⅲ (P<0.05; and P<0.01, respectively). There were strong correlations between the degree of tubulointerstitial lesions and the expression of P selectin on tubular epithelium or within interstitium (rs=0.395 and rs=0.337, P<0.01).Furthermore, P slectin messenger RNA (mRNA) signals were also detected in the cytoplasm of glomerulus, tubular epithelial cells, intertitium and vascular endothelium. Conclusions P selectin might mediate intraglomerular platelets aggregation, activation and leukocyte accumulation in the early stages of human proliferative glomerulonephritis. The up regulation of P selectin in interstitium was associated with interstitial fibrosis and tubular atrophy and may contribute to the progression of glomerulonephritis.Objective To examine the relationship between P selectin expression and human glomerulonephritis. Methods P selectin expression was investigated in renal biopsies of glomerulonephritis patients (n=133) by immunohistochemical and in situ hybridization analyses. They were divided into three groups based on the degrees of cells proliferation or sclerosis. Group Ⅰ: histological lesions in glomeruli are mild (n=23); Group Ⅱ: glomerular cells proliferation is prominent (n=91); Group Ⅲ: glomerular sclerosis is severe (n=19). The Kruskal Wallis test, Chisquare test and Spearman's correlation were used in our study. Results In normal controls, renal P selectin expression was negative (n=10). In glomerulonephritis, the up regulated P selectin expression on tubular epithelium was significantly higher than that on glomeruli and interstitium (P<0.01). P selectin was expressed predominantly on platelets in glomeruli, and glomerular P selectin expression was more significantly up regulated in Group Ⅱ than in Group Ⅰ or Group Ⅲ (P<0.05; and P<0.01, respectively). There were strong correlations between the degree of tubulointerstitial lesions and the expression of P selectin on tubular epithelium or within interstitium (rs=0.395 and rs=0.337, P<0.01).Furthermore, P slectin messenger RNA (mRNA) signals were also detected in the cytoplasm of glomerulus, tubular epithelial cells, intertitium and vascular endothelium. Conclusions P selectin might mediate intraglomerular platelets aggregation, activation and leukocyte accumulation in the early stages of human proliferative glomerulonephritis. The up regulation of P selectin in interstitium was associated with interstitial fibrosis and tubular atrophy and may contribute to the progression of glomerulonephritis.

分 类 号：R692.31[医药卫生—泌尿科学]