应用RAPD标记构建马尾松单株树遗传连锁图谱  

The Construction of Genetic Linkage Mapping for Individual of Pinus massoniana by RAPDs Marker

作　　者：郑先武[1] 陈伯望[1] 唐谦[1] 魏令波[1] 

机构地区：[1]中国林业科学研究院林业研究所,中国科学院发育生物研究所

出　　处：《应用基础与工程科学学报》1997年第2期181-186,共6页Journal of Basic Science and Engineering

基　　金：国家自然科学基金

摘　　要：利用马尾松（Ｐｉｎｕｓｍａｓｓｏｎｉａｎａ）崇义群体Ｃ－１６号单株上４０粒种子的胚乳为作图群体材料，用随机扩增多态性ＤＮＡ（ＲＡＰＤ）方法构建马尾松单株遗传连锁图谱，从被步筛选的８０个引物中，得到１６个具有多态性产物的引物，对这１６种引物进行Ｍｅｎｄｅｌｉａｎ１：１分离检测（卡方检验，Ｐ＜０．０５），我们得到符合Ｍｅｎｄｅｌｉａｎ１：１分离比例的ＲＡＰＤｓ标记４９个．通过对４９个标记的连锁关系进行分析，其中２９个标记分布在１２个连锁群上，总图距为４８３．３ｃＭ，平均图距为２８．４２ｃＭ，２０个标记没有连锁到任何连锁群上，需要继续进行大量标记的连续分析，构建完善的高密度的遗传图谱，使之能够对数量性状位点ＱＴＬｓ进行定位，从而进行分子标记辅助选择育种（ＭＡＳ）．The genetic linkage mapping for individual of Pinus massoniana was con- structed by using the megagametophyte tissue of seeds from individual C- 1 6 of Cong Yi population and the method of Random Amplified Polymorphic DNA (RAPD) .80 primers were screened and 16 primers that can reveal polymorphism were available. According to the test of goodness of fit to a 1:1 Mendelian ratio of segregating by Chi square analysis at the 0.05 singnificance level, we have de- termined 49 RAPD Markers, of which 29 were mapped to 12 linkage groups, cov- ered a total of 483. 3cM, and averaged 28. 42cM, while the remaining 20 were u,.- linked. We will carry out the research on the marker assisted-selection (MAS) based on genetic linkage mapping and the mapping of quantative trait loci (QTLs) .

关 键 词：马尾松 遗传连锁图谱 随机扩增多态性DNA(RAPD) 数量性状位点QTLs 

分 类 号：S718.46[农业科学—林学] S791.248