长柄链格孢(Alternaria longipes)和链格孢(A.alternata)的RAPD分析  

Phylogentic Relationships Between Alternaria longipes and A. alternata Revealed by RAPD

作　　者：陈伟群[1] 张天宇[2] 

机构地区：[1]西北农业大学植保系,杨陵712100 [2]山东农业大学植保系

出　　处：《中国烟草学报》1997年第1期30-35,共6页Acta Tabacaria Sinica

基　　金：中科院微生物所真菌地在系统学开放实验室资助

摘　　要：对来自中国及亚、非、欧三大洲5个国家的10个烟草赤星病菌（Alternarialongies）及5个链格孢菌（A．alternata）标准菌株用6种随机引物（OPR01、OPR12、OPR13、OPR14、OPR15、OPK19）进行了基因组DNA随机扩增多态性分析，对RAPD带型所进行的聚类分析结果表明，所有参试菌株之间相似性很高（90．3％～94．7％），其系统聚类关系与寄主和地理来源没有相关性，证明A．longipes与A．alternata亲缘关系很近，建议将A．alternata确定为烟草赤星病菌的学名。Ten isolates of Alternaria longipes and five isolates of A. alternata from China,Greece, U. K., Zambia, Cyprus and lndia were characterized by RAPD (Radomly Amplified Polymorphic DNA ) assay with six 10 - mer prirners (OPR01, OPR12, OPR13,OPR14, OPR15,OPK19). Computer analysis of the data showed a low genetic diversity between the two species and among the isolates. All the isolates were closely ralated, the similarity coefficients were 88. 9%~ 94. 7%- Isolates from widely separated geographical locations and different hosts/substrates exibit nearly identical or very similar RAPD profiles. It is recommended that A. alternata should be used as the valid name of the pathogen of brown spot lesion of tobacco.

关 键 词：链格孢 长柄链格孢 RAPD 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]