Immobilization of microorganisms using carrageenan gels coated with chitosan and application to biodegradation of 4-chlorophenol  

作　　者：Wang Jianlong Li Ping Shi Hanchang Qian Yi China Zhou Ding 

机构地区：[1]Environment Simulationand Pollution Control State Key Joint Laboratory,Department of Environmental Engineering,Tsinghua University,Beijing 100084 [2] Department of Applied Chemistry,Harbin Institute of Technology,Harbin150001,China

出　　处：《Journal of Environmental Sciences》1997年第3期30-34,共5页环境科学学报(英文版)

摘　　要：A new cell immobilization method based on the replacement of KCl by KCl+chitosan as the gelling agent was developed. The experimental results showed that through addition of chitosan into gelling agent, the mechanical strength and the thermal stability of the carrageenan gel were greatly improved. The new immobilization method was used to entrap a chlorophenol degrading microorganism. The immobilized microbial cells were applied for chlorophenol biodegradation. The experiments demonstrated that immobilized cells exhibit a higher bioactivity in the degradation of chlorophenol than free cells.A new cell immobilization method based on the replacement of KCl by KCl+chitosan as the gelling agent was developed. The experimental results showed that through addition of chitosan into gelling agent, the mechanical strength and the thermal stability of the carrageenan gel were greatly improved. The new immobilization method was used to entrap a chlorophenol degrading microorganism. The immobilized microbial cells were applied for chlorophenol biodegradation. The experiments demonstrated that immobilized cells exhibit a higher bioactivity in the degradation of chlorophenol than free cells.

关 键 词：CARRAGEENAN CHITOSAN IMMOBILIZATION 4  chlorophenol biodegradation. 

分 类 号：X592[环境科学与工程—环境工程]