Chromosome G-banding in SituHybridization of RFLP Marker umc58 Linked with the Gene hm1 Dictating Helminthosporium carbonum Susceptibility1 in Maize  

作　　者：Song Yunchun Ren Nan Mao Ninghui Liu Lihua 

机构地区：[1]College of Life Sciences,Wuhan University,Wuhan430072,China

出　　处：《Wuhan University Journal of Natural Sciences》1997年第2期99-103,共5页武汉大学学报（自然科学英文版）

摘　　要：The technique of simultaneous G banding and in situ hybridization has been developed in plants for the first time.Using this technique.RFLP marker umc58 closely linked with the hm1 gene dictating Helminthosporium carbonum susceptibility1 was localized onto 1L3(chromosome 1,long arm,the third band from the centromere to the end of the arm),5L5 and 9L5.Theresults demonstrated that umc58 was a tripli cated sequence.It was deduced that umc58 probably was in a duplicated region that includes a part of Helminthosporium carbonum susceptibility genes(hm1 and hm2),as the hybridization sites of umc58 in chro mosomes 1 and 9 were those at which the genes localize.The techniques of simultaneous G banding and ISH in plants are discussed.

关 键 词：In situ hybridization chromosome G banding MAIZE RFLP markers 

分 类 号：Q7[生物学—分子生物学]