血小板低温保存实验与临床应用研究  

The Experiment and Clinical Application of Cryopreserved platelets

作　　者：刘景汉[1] 王青梅[1] 李茨芬 殷宗健[1] 

机构地区：[1]中国人民解放军总医院输血科,北京100853

出　　处：《生物医学工程与临床》1997年第1期45-48,共4页Biomedical Engineering and Clinical Medicine

摘　　要：目的:探索并建立低温保存血小板实验方法和技术,以解决临床用血小板难的问题。方法:以终浓度为4％～5％DMSO,于—80℃条件下保存从新鲜血液中分离提取的、仍保持较高活性的血小板为对象,进行低温生物学研究,并做了853例患者输注临床观察。结果:血小板保存6～16个月,采用联合诱导剂检测血小板功能,其血小板聚集功能和粘附率保持在新鲜血小板水平的90％以上,低渗休克反应恢复率为52％±10.1％,KPTT、Ⅶ因子R:Ag及正因子有效性亦保持正常,临床输注后血小板数明显升高,获得满意的止血效果。结论:DMSO系细胞内超低温保护剂,于—80℃条件下仍能有效地保存血小板活性;输注含有DMSO的血小板,对人体是安全的,并具有提高血小板的作用和良好的止血功能。Purpose:experiments were performed in order to solve the problems in the long term cyopreserved platelets can be available to the patients. Methods: The platelets separated from fresh blood of 853 cases were frozen at -80℃ in freezer immediately at the final concentration of 4 %~5 % DMSO. Result: 6~16 months after cryopreservation, the aggregation function of platelets were tested with combined reagents(inducer). Thier level of aggregation and adhension rate were 90% higher than those of fresh platelets. The results of KPTT,the activity of faster Ⅷ and factor Ⅲ were all at the normal range. After infusion of these cryopreserved platelets, the platelet count of patients increased markedly and hemorrhage events were all controlled effectively. Conclusion: DMSO is a kind of intracellular cryoprotectent, which can maintain to activity of cryopreseve platelet at -80℃. Infusion of cryopreserved platelet containing only a litte amout of DMSO will not do obviously harm to human body,the function of hemostasis was observed.

关 键 词：DMSO 冷冻保存血小板 输血 

分 类 号：R318.52[医药卫生—生物医学工程]