大鼠烧伤后血浆内皮素1含量变化及其意义  

The change of plasma endothelin-1 concentration in burned rats and its significance

作　　者：李元义[1] 方之扬[1] 陈玉林[1] 葛绳德[1] 刘世康[1] 

机构地区：[1]第二军医大学长海医院烧伤科

出　　处：《第二军医大学学报》1996年第S1期34-36,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金

摘　　要：目的：探讨内皮素１（ＥＴ－１）对烧伤后血管通透性的影响。方法：大鼠３０％体表面积Ⅲ度烫伤后，用放射免疫分析法测定血浆ＥＴ－１的浓度变化；烫伤后即刻及１，２，３ｈ分别静注ＥＴ－１特异性抗血清（滴度１：５０００）１ｍｌ／ｋｇ，观察烫伤大鼠平均动脉压和［１２５Ｉ］－白蛋白分布的变化。结果：大鼠烫伤后，血浆ＥＴ－１浓度很快升高，伤后４ｈ达高峰，２４ｈ仍明显高于正常对照，４８ｈ降至正常水平。延迟复苏大鼠的ＥＴ－１浓度，虽然与立即复苏组没有显著差别，但各时相点的ＥＴ－１浓度均高于立即复苏组。静注ＥＴ－１抗血清组的大鼠平均动脉压与对照组无显著差别，但［１２５Ｉ］－白蛋白在心、肺、空肠和骨胳肌组织的分布明显少于对照组。结论：烧伤后血浆ＥＴ－１参与烧伤后血管通透性增加的发生发展。Objective:To investigate the pathogenetic role of endothelin-1 (ET-1) in burn shock. Methods:The plasma ET-1 concentration in rats with 30% third degree burn was investigated by radioim munoassay. and following injection of ET-1 antiserum immediately and at 1 , 2, 3 h postburn,the mean arterial pressure(MAP) and distribution of [125I]-albumin in burned rats were measured. Results: The plasma ET-1 concentration in burned rats increased further,with its peak at 4 h postburn. Thereafter ETl concentration decreased but still increased at 24 h , and returned to baseline level at 48 h postburn. Every value was higher in the delayed resuscitation group than that in the immediate resuscitation group. although there was no significant difference within the two groups. Injection of ET-1 antiserum had no marked effect on MAP. but significantly decreased the distribution of [125I]-albumin in the heart . lung,jejunum and muscle in burned rats. Conclusion: Plasma ET-1 level is one of the important pathogenetic factors of vascular permeability enhancement postburn.

关 键 词：内皮素1 血管通透性 灼伤 大鼠 

分 类 号：R644[医药卫生—外科学]