茁霉多糖酶产生菌株A_(3345)的选育  

Selection of Pullulanase Producing Strain A 3345

作　　者：王义良[1] 张添元[1] 罗进贤[1] 

机构地区：[1]中山大学生命科学学院

出　　处：《中山大学学报（自然科学版）》1996年第S2期54-57,共4页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省自然科学基金

摘　　要：报道以产茁霉多糖酶的产气气杆菌（Ａｅｒｏｂａｃｔｅｒａｅｒｏｇｅｎｅｓ）为出发菌株，采用亚硝基胍、紫外线、亚硝酸等交叉或复合处理的方法，以突变株中筛选出一株产茁霉多糖酶活力较高的Ａ３３４５菌株．摇瓶试验表明该菌株在藤尾培养基中，３０℃发酵４ｄ，酶活力在７．５×１０５Ｕ／Ｌ以上．比原出发菌株提高１１０％．该酶反应的最适ＰＨ５．２～６．４。A Aerobacter aerogenes mutant producing high pullulanas eactivity was selected by reciprocal or mixed treatment of the parental strain with NTG; U.V and HNO 2. The pullulanase activity of the selected strain is 750 u/mL in Teng Wei (D M) medium (A) after 4 days incobation at 30℃, which is 110% higher than the parental strain. The optimal pH and temperature is 5 2～6 4 and 50℃ respectively.

关 键 词：茁霉多糖酶 产气气杆菌 诱变 

分 类 号：Q920.1[生物学]