人类精子核的研究──100例不育病人精子碱性核蛋白的分析  

THE STUDIES ON HUMAN SPERM NUCLEUS-NALYSIS OF SPERM NUCLEAR BASIC PROTEINS IN 100 INFERTILE MEN

作　　者：费仁仁[1,2] 陈晖[1,2] 熊惠芳 万启智[1,2] 冯建芳 章静波 薛社普[1,2] 

机构地区：[1]中国医学科学院药物研究所 [2]成都计划生育指导所,中国医学科学院基础医学研究所

出　　处：《解剖学报》1996年第3期256-258,共3页Acta Anatomica Sinica

摘　　要：对１００例随机选择的不育病人精子核碱性蛋白质进行分析，结果表明有５４％病人的碱性蛋白异常，并可将其分为３类：１．组蛋白－精核蛋白（ｈｉｓｔｏｎｅ－ｐｒｏｔａｍｉｎｅ）取代反应受阻，占总病历２３％；２．３种精核蛋白（ＨＰ１，ＨＰ２，ＨＰ３）比例异常，占总病历３０％；３．精核蛋白完全缺失，占总病历１％。文中还对精核蛋白与生育力的关系以及精子碱性核蛋白分析在不育病人诊断中的重要性进行了讨论。Sperm nuclear basic proteins were extracted from 100 infertile men at random.TH(total histones) /TP(total protamines)and HP1/HP2 +HP3 ratios were determined by scanning microdensitometry following electrophoresis of total nuclear basic protein in polyacrylamide gels. The results in dicated that 46%of total cases had normal sperm nuclear basic proteins and 54%had aberrant ones.According to the data,we suggested to classify aberrant cases into 3 groups;1.histone-to-protamine replacement reaction was interrupted(23%);2.HP1/HP2+HP3 ratio was altered(30%,6%were complete selective absence of HP2+HP3);3.complete absence of protamines(1%).The relationship between fertility and protamine and the imporiance of analysis of sperm nuclear basic proteins in diag nosis of infertility was discussed.

关 键 词：精子核 精核蛋白 组蛋白-精核蛋白取代反应 不育 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]